Human Progranulin/PGRN Antibody (296628)

R&D Systems | Catalog # MAB2420

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunocytochemistry, ELISA Detection, ELISA Development, ELISA Development (Detection), FRET

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 296628
View all Progranulin/PGRN Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Progranulin/PGRN
Thr18-Leu593
Accession # P28799

Specificity

Detects recombinant human Progranulin/PGRN in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Progranulin/PGRN is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Progranulin/PGRN Antibody (296628)

Detection of Human Progranulin/PGRN by Western Blot

Detection of Human Progranulin/PGRN by Western Blot

Specificity of PGRN antibodies and detection of multiple endogenous GRNs. A, Whole-cell lysates from HAP1 WT and HAP1 PGRN KO cells, either reduced (+TCEP) or nonreduced (-TCEP), were analyzed for PGRN antibody specificity by immunoblot. The bottoms of the blots (<15 kDa) were exposed longer to reveal levels of endogenous GRNs. B, Whole-cell lysates from HEK293T WT and N-TAP PGRN stable overexpressing (PGRN) cells were analyzed as in A by immunoblot. C, Representative immunofluorescent images of HAP1 PGRN KO and WT cells stained with the corresponding PGRN antibodies as in A and B. Scale bar, 10 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28828399), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Progranulin/PGRN by Western Blot

Detection of Human Progranulin/PGRN by Western Blot

Specificity of PGRN antibodies and detection of multiple endogenous GRNs. A, Whole-cell lysates from HAP1 WT and HAP1 PGRN KO cells, either reduced (+TCEP) or nonreduced (-TCEP), were analyzed for PGRN antibody specificity by immunoblot. The bottoms of the blots (<15 kDa) were exposed longer to reveal levels of endogenous GRNs. B, Whole-cell lysates from HEK293T WT and N-TAP PGRN stable overexpressing (PGRN) cells were analyzed as in A by immunoblot. C, Representative immunofluorescent images of HAP1 PGRN KO and WT cells stained with the corresponding PGRN antibodies as in A and B. Scale bar, 10 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28828399), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Progranulin/PGRN Antibody (296628)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Progranulin (Catalog # 2420-PG), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Progranulin (Catalog # 2420-PG) under non-reducing conditions only

Reviewed Applications

Read 4 reviews rated 4 using MAB2420 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Progranulin/PGRN

Progranulin, also known as acrogranin, PC cell-derived growth factor (PCDGF) and epithelin/granulin precursor, is a ubiquitously expressed, 88 kDa, secreted glycoprotein (1‑3). Structurally, it does not belong to any of the well-established growth factor families (4). Human Progranulin is 593 amino acids (aa) in length and contains a 17 aa signal sequence and 5 potential sites for N-linked glycosylation. It has a highly repetitive organization, containing seven tandem copies of a 55‑57 aa consensus motif that contains 12 conserved cysteine residues: VxCx5-6Cx5CCx8CCx6CCxDx2HCCPx4Cx5-6Cx2 (1). There is one alternate splice form for human Progranulin. This has a deletion of aa corresponding to aa 377‑531 of the standard form. Progranulin is secreted as a full length form (2, 4), and may undergo proteolysis leading to the release of numerous peptides made from the seven tandem repeats, called the granulins (5‑7). Human Progranulin shares 75% aa sequence identity with mouse and rat Progranulin. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development, and pathological processes (4). It has been isolated as a differentially expressed gene during mesothelial differentiation (8), macrophage development (9), the development of rheumatoid arthritis and osteoarthritis (10), sexual differentiation of the brain (11), and has also been shown to be a mediator of cartilage proliferation and of wound response and tissue repair (4, 12‑13). High levels of Progranulin expression have been found to be associated with several human cancers and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma, and multiple myeloma
(4‑5, 12, 14‑19). In addition, mutations in the Progranulin gene are a cause of frontotemporal dementia, and increased expression of Progranulin is seen in activated microglia in many neurodegenerative diseases including Creutzfeldt-Jakob disease, motor neuron disease and Alzheimer's disease (20). Mutations in Progranulin causing neurodegenerative disease indicate that Progranulin is important for neuronal survival (20).

References

  1. Plowman, G.D. et al. (1992) J. Biol. Chem. 267:13073.
  2. Zhou, J. et al. (1993) J. Biol. Chem. 268:10863.
  3. Liu, Y. et al. (2007) BMC Cancer 7:22.
  4. Xu, K. et al. (2007) J. Biol. Chem. 282:11347.
  5. Davidson, B. et al. (2004) Cancer 100:2139.
  6. Zanocco-Marani, T. et al. (1999) Cancer Res. 59:5331.
  7. Lu, R. and G. Serrero (2000) Proc. Natl. Acad. Sci. U.S.A. 97:3993.
  8. Sun, X. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:510.
  9. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:727.
  10. Justen, H.P. et al. (2000) Mol. Cell Biol. Res. Commun. 3:165.
  11. Suzuki, M. and M. Nishiahara (2002) Mol. Genet. Metab. 75:31.
  12. He, Z. et al. (2003) Nat. Med. 9:225.
  13. Zhu, J. et al. (2002) Cell 111:867.
  14. He, Z. and A. Bateman (2003) J. Mol. Med. 81:600.
  15. Bateman, A. et al. (1990) Biochem. Biophys. Res. Commun. 173:1161.
  16. Gonzalez, E.M. et al. (2003) J. Biol. Chem. 278:38113.
  17. Jones, M.B. et al. (2003) Gynecol. Oncol. 88:S136.
  18. Wang, W. et al. (2003) Clin. Cancer Res. 9:2221.
  19. Zhang, H. and G. Serrero (1998) Proc. Natl. Acad. Sci. U.S.A. 95:14202.
  20. Baker, M. et al. (2006) Nature 442:916.

Alternate Names

Acrogranin, GEP, GP88, GRN, PCDGF, PEPI, PGRN, Proepithelin

Entrez Gene IDs

2896 (Human); 14824 (Mouse)

Gene Symbol

GRN

UniProt

P28799 (Human)

Additional Progranulin/PGRN Products

Product Documents for Human Progranulin/PGRN Antibody (296628)

Certificate of Analysis

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Product Specific Notices for Human Progranulin/PGRN Antibody (296628)

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human Progranulin/PGRN Antibody (296628)

Customer Reviews for Human Progranulin/PGRN Antibody (296628) (4)

4 out of 5
4 Customer Ratings
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  • Human Progranulin/PGRN Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Human Progranulin/PGRN Antibody (296628) MAB2420
  • Human Progranulin/PGRN Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Progranulin/PGRN Antibody (296628) MAB2420
  • Human Progranulin/PGRN Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018
  • Human Progranulin/PGRN Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018

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