Human Proteinase 3/Myeloblastin/PRTN3 Antibody (684022)

R&D Systems | Catalog # MAB61341

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 684022
View all Proteinase 3/Myeloblastin/PRTN3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Proteinase 3/Meyeloblastin/PRTN3
Ala26-Arg249
Accession # P24158

Specificity

Detects human Proteinase 3/Meyeloblastin/PRTN3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Proteinase 3/Myeloblastin/PRTN3 Antibody (684022)

Proteinase 3/Myeloblastin/PRTN3 antibody in Human Granulocytes by Immunocytochemistry (ICC).

Proteinase 3/Myeloblastin/PRTN3 in Human Granulocytes.

Proteinase 3/ Myeloblastin/PRTN3 was detected in immersion fixed human granulocytes using Mouse Anti-Human Proteinase 3/ Myeloblastin/PRTN3 Monoclonal Antibody (Catalog # MAB61341) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Proteinase 3/Myeloblastin/PRTN3 Antibody (684022)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human granulocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Proteinase 3/Myeloblastin/PRTN3

Leukocyte proteinase 3 (PRTN3 or PR3), also called Myeloblastin (MBN) or NP-4, is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human MBN proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C-terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human MBN shares 68% aa sequence identity with mouse MBN.

Alternate Names

ACPA, AGP7, C-ANCA, MBN, MBT, Myeloblastin, NP4, PRTN3

Entrez Gene IDs

5657 (Human); 19152 (Mouse); 314615 (Rat)

Gene Symbol

PRTN3

UniProt

P24158 (Human)

Additional Proteinase 3/Myeloblastin/PRTN3 Products

Product Documents for Human Proteinase 3/Myeloblastin/PRTN3 Antibody (684022)

Certificate of Analysis

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Product Specific Notices for Human Proteinase 3/Myeloblastin/PRTN3 Antibody (684022)

For research use only

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