Human RUNX1/CBFA2 Antibody

Catalog #: AF2399 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2399
AF2399-SP

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Detection of Human RUNX1/CBFA2 by Western Blot.

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All RUNX1/CBFA2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human RUNX1/CBFA2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human RUNX1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RUNX1
Gly190-Tyr453
Accession # Q01196

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RUNX1/CBFA2 by Western Blot. Western blot shows cytoplasmic and nuclear extracts from Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human RUNX1/CBFA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2399) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for RUNX1/CBFA2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human RUNX1/CBFA2 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for RUNX1/ CBFA2 at approximately 59 kDa (as indicated) using 20 µg/mL of Goat Anti-Human RUNX1/CBFA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2399) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RUNX1/CBFA2

RUNX1, also known as CBFA2, is one of three members of the Runt-related family. The RUNX/CBFA proteins form the alpha subunits that combine with the beta subunit (CBFB) to form the heterodimeric transcription factors. RUNX/CBFA plays important roles in hematopoiesis and may regulate the generation of hematopoietic stem cells from hematogenic endothelial cells. Deregulated expression of CBFA2/RUNX1 is associated with malignant diseases of the hematopoietic system. Human and mouse RUNX/CBFA share 95% amino acid sequence homology.

Long Name

Runt-related Transcription Factor 1

Entrez Gene IDs

861 (Human); 12394 (Mouse); 50662 (Rat)

Alternate Names

Acute myeloid leukemia 1 protein; AML1; AML1acute myeloid leukemia 1; AML1-EVI-1; CBFA2; CBFA2acute myeloid leukemia 1 protein (oncogene AML-1), core-binding factor, alphasubunit10AMLCR1; Core-binding factor subunit alpha-2; EVI-1; PEBP2A2; runt domain, alpha subunit 2; runt-related transcription factor 1; RUNX1; SL3/AKV core-binding factor alpha B subunit