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Human S1P2/EDG-5 Antibody

Catalog #: MAB3649
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB3649 has been discontinued.
View all S1P2/EDG-5 products.
Detection of S1P2/EDG-5 in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP by Flow Cytometry.
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Human S1P2/EDG-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human S1P2/EDG-5. Stains human S1P2/EDG-5 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 368510
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human S1P2/EDG-5
Met1-Val353
Accession # AAC98919
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<sub>2</sub>/EDG-5 antibody in HEK293 Human Cell Line Transfected with Human S1P<sub>2</sub>/EDG-5 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P2/EDG-5 in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 Monoclonal Antibody (Catalog # MAB3649) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P2/EDG-5

S1P2 (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a 353 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P2 show 82.5% aa identity with mouse S1P2 and 92% aa identity with human EDG-3, the most closely related family member. Both are expressed predominantly in the lung, heart, kidney, liver, spleen, thymus, testis and brain, mediating both mitogenic and anti-apoptotic effects.

Long Name
Endothelial Differentiation Gene 5
Entrez Gene IDs
9294 (Human); 14739 (Mouse); 29415 (Rat)
Alternate Names
AGR16; EDG5; EDG-5; endothelial differentiation G-protein coupled receptor 5; endothelial differentiation, sphingolipid G-protein-coupled receptor, 5; GPCR13; GPCR18; H218; LPB2; S1P receptor 2; S1P receptor EDG5; S1P receptor Edg-5; S1P2; S1PR2; sphingosine 1-phosphate receptor 2; sphingosine 1-phosphate receptor Edg-5; sphingosine-1-phosphate receptor 2

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Citation for Human S1P2/EDG-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. S1P1 expression is controlled by the pro-oxidant activity of p66Shc and is impaired in B-CLL patients with unfavorable prognosis.
    Authors: Capitani, Nagaja, Patrussi, Laura, Trentin, Livio, Lucherini, Orso Mar, Cannizzaro, Enrica, Migliaccio, Enrica, Frezzato, Federica, Gattazzo, Cristina, Forconi, Francesc, Pelicci, Piergius, Semenzato, Gianpiet, Baldari, Cosima T
    Blood, 2012-10-01;120(22):4391-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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