Human Semaphorin 4B Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5485R Datasheet / COA / SDS

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AF5485R-100UG

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Human Semaphorin 4B Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Semaphorin 4B in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse Semaphorin 4B is observed and less than 1% cross‑reactivity with recombinant human (r

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 4B isoform 1
Leu39-Glu712
Accession # Q9NPR2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Semaphorin 4B

Semaphorin 4B (Sema4B; also SemaC) is a 95‑100 kDa, class IV member of the Semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids (aa) in length. It contains a 674 aa extracellular region (aa 39‑832) that is characterized by one Sema domain (aa 65‑502), a PSI region (aa 520‑574), and an Ig-like C2-type domain (aa 599‑658). There is one potential soluble splice variant that shows a 29 aa substitution for aa 707‑832. Over aa 39‑712, human Sema4B shares 86% aa identity with mouse Sema4B.

Entrez Gene IDs

10509 (Human); 20352 (Mouse); 293042 (Rat)

Alternate Names

KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B; MGC131831; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B; SEMA4B; SEMAC; Semaphorin 4B; semaphorin-4B; SemC