Human Serpin B2 Antibody (930119)

R&D Systems | Catalog # MAB85501

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 930119
View all Serpin B2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Serpin B2
Glu2-Ala65, Ala99-Pro415
Accession # P05120

Specificity

Detects human Serpin B2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Serpin B2 Antibody (930119)

Western Blot Show Human Serpin B2 Antibody Specificity Using Knockout Cell Line.

Western Blot Show Human Serpin B2 Specificity Using Knockout Cell Line.

Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Serpin B2 knockout K562 cell line (KO) untreated (-) or treated (+) with 300nM PMA for overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 40 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. HSP60 (Catalog # MAB1800) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Serpin B2 antibody by Western Blot.

Detection of Human Serpin B2 by Western Blot.

Western blot shows lysates of HEK001 human epidermal keratinocyte cell line (untreated) and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Serpin B2 antibody by Simple WesternTM.

Detection of Human Serpin B2 by Simple WesternTM.

Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. Specific bands were detected for Serpin B2 at approximately 45 & 50 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Serpin B2 Antibody (930119)

Application
Recommended Usage

Knockout Validated

2 µg/mL
Sample: K562 human chronic myelogenous leukemia parental cell line and Serpin B2 knockout K562 cell line treated ith PMA

Simple Western

20 µg/mL
Sample: U937 human histiocytic lymphoma cell line treated with PMA

Western Blot

0.05 µg/mL
Sample: HEK001 human epidermal keratinocyte cell line (untreated) and U937 human histiocytic lymphoma cell line treated with PMA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin B2

Serpin B2, also known as Plasminogen Activator Inhibitor 2 (PAI-2), is a 415 amino acid serine-type endopeptidase inhibitor with high expression in blood, vasculature and placenta. Its expression is upregulated in pregnancy and in activated monocytes/macrophages by a wide range of viral, bacterial and parasitic agents. Serpin B2 is upregulated in monocytes from HIV-1 infected patients, and is thought to modulate Th1/Th2 responses. Serpin B2/PAI-2 colocalizes with CHL1 and Vitronectin and mediates neurite outgrowth during post-natal brain development. Inhibition of uPA by SerpinB2 in tumors is associated with a favorable prognosis.

Alternate Names

PAI2, PLANH2, Urokinase Inhibitor

Entrez Gene IDs

5055 (Human); 18788 (Mouse); 60325 (Rat)

Gene Symbol

SERPINB2

UniProt

P05120

Additional Serpin B2 Products

Product Documents for Human Serpin B2 Antibody (930119)

Certificate of Analysis

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Product Specific Notices for Human Serpin B2 Antibody (930119)

For research use only

Citations for Human Serpin B2 Antibody (930119)

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