Human SHIP2 Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB5389AFP647

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 737935
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Product Specifications

Specificity

Detects human SHIP2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse SHIP2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human SHIP2 Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SHIP2

SHIP2 (SH2 domain-containing inositol 5-phosphatase 2; also inositol polyphosphate phosphatase-like 1) is a 160 kDa member of the phosphatidylinositol/PtdIns 5-phosphatase family of enzymes.  It is widely expressed and negatively regulates PI3 kinase pathways by hydrolyzing the 5-phosphate of PtdIns-3,4,5-triphosphate.  Human SHIP2 is 1258 amino acids (aa) in length.  It contains one SH2 domain (aa 21-117), a Pro-rich region with an SH3 binding site (aa 935-1105), and a SAM (or sterile alpha -motif) domain that binds ARAP3 (aa 1196-1258). Phosphorylation is known to occur on Tyr986, 1162 and 1358, and on Thr958. There is an alternate start site at Met243 and a second splice variant that shows a 40 aa substitution for aa 1228-1258. Over aa 1106-1258, human SHIP2 shares 95% aa identity with mouse SHIP2.

Long Name

SH2-containing Inositol-5'-Phosphatase 2

Alternate Names

INPPL1

Entrez Gene IDs

3636 (Human); 16332 (Mouse); 65038 (Rat)

Gene Symbol

INPPL1

UniProt

Additional SHIP2 Products

Product Documents for Human SHIP2 Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human SHIP2 Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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