Human Siglec-16 Antibody (706030)

R&D Systems | Catalog # MAB68192

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 706030
View all Siglec-16 Primary Antibodies »

Product Specifications

Immunogen

Human Siglec-16 peptide
Accession # A6NMB1

Specificity

Detects human Siglec-16 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Siglec-16 Antibody (706030)

Detection of Siglec-16 in HEK293 Human Cell Line Transfected with Human Siglec-16 and eGFP by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with human Siglec-16 and eGFP was stained with (A) Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68192) or (B) Mouse IgG1 Isotype Control Antibody (MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).Staining was done using our Staining Membrane-associated Proteins protocol.

Detection of Siglec‑16 in CHO cells transfected with Human Siglec-16 or irrelevant Transfectant cells by Flow Cytometry.

CHO cells transfected with human Siglec-16 (filled histogram) or irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human Siglec-16 Monoclonal Antibody (Catalog # MAB68192), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

Applications for Human Siglec-16 Antibody (706030)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Siglec-16 and eGFP

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: CHO cells transfected with human Siglec-16 or irrelevant transfectant

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-16

Siglec-16 (Sialic acid-binding Ig-like lectin 16) is a 58-60 kDa member of the CD33-related SIGLEC family of proteins. It is expressed on macrophages and microglia, and based on Siglec-11, likely binds to an alpha 2,8-linked sialic acid motif. Although Siglec-16 is assumed to have arisen from a Siglec-11 gene duplication and conversion, it is not an inhibitory receptor but an activating one, and possesses a transmembrane (TM) Lys that interacts with DAP12. Mature human Siglec-16 is a 465 amino acid (aa) type I TM glycoprotein. It contains a 418 aa extracellular region (aa 17-434) that shows one V-type (aa 19-122) plus three C2-type (aa 147-424) Ig‑like domains, and a short 26 aa cytoplasmic tail. Notably, Siglec-16 exists as a pseudogene in approximately 50% of surveyed population. There is no Siglec-16 counterpart in either rodent or distant primate such as Rhesus monkey. The extracellular domain (ECD) of human Siglec-16 shares 94% aa identity with the ECD of human Siglec-11.

Long Name

Sialic Acid Binding Ig-like Lectin 16

Alternate Names

Siglec16, SIGLECP16

Entrez Gene IDs

400709 (Human)

Gene Symbol

SIGLEC16

UniProt

A6NMB1 (Human)

Additional Siglec-16 Products

Product Documents for Human Siglec-16 Antibody (706030)

Certificate of Analysis

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Product Specific Notices for Human Siglec-16 Antibody (706030)

For research use only

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