Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 752219
View all Sin1/MAPKAP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Sin1/MAPKAP1
Lys408-Gln522
Accession # Q9BPZ7

Specificity

Detects human Sin1/MAPKAP1 in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Sin1/MAPKAP1 Antibody

Detection of Human Sin1/MAPKAP1 antibody by Western Blot.

Detection of Human Sin1/MAPKAP1 by Western Blot.

Western blot shows lysates of human heart tissue and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Sin1/MAPKAP1 Monoclonal Antibody (Catalog # MAB8168) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Sin1/MAPKAP1 at approximately 59-63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Sin1/MAPKAP1 Antibody

Application
Recommended Usage

Western Blot

0.2 µg/mL
Sample: Human heart tissue and human kidney tissue

Reviewed Applications

Read 1 review rated 4 using MAB8168 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sin1/MAPKAP1

MAPKAP1 (mitogen-activated protein kinase (MAPK) associated protein-1), also called Mip1 (MAPK interacting protein 1) or Sin1 (stress-activated protein kinase (SAPK) interacting 1), is a widely expressed 59-65 kDa cytoplasmic phosphoprotein. MAPKAP1 binds to inactive forms of signaling molecules such as MEKK2 and Ras. Alternate mRNA splicing potentially creates isoforms of 323, 330, 372, 475, 486 and 522 amino acids (aa) in humans. The human MAPKAP1 region used as an immunogen is included in all except the smallest isoform, and shares 96% aa sequence identity with mouse and rat MAPKAP1.

Long Name

Mitogen-Activated Protein Kinase Associated Protein 1

Alternate Names

JC310, MAPKAP1, MIP1

Entrez Gene IDs

79109 (Human); 227743 (Mouse); 296648 (Rat)

Gene Symbol

MAPKAP1

UniProt

Q9BPZ7

Additional Sin1/MAPKAP1 Products

Product Documents for Human Sin1/MAPKAP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Sin1/MAPKAP1 Antibody

For research use only

Related Research Areas

Autophagy

Citations for Human Sin1/MAPKAP1 Antibody

Customer Reviews for Human Sin1/MAPKAP1 Antibody (1)

4 out of 5
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  • Human Sin1/MAPKAP1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipose tissue
    Species: Mouse
    Verified Customer | Posted 10/23/2019
    Mouse white adipose tissue was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody MAPKAP1 diluted 1:1000 in 5% BSA 2, before incubation with anti mouse secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.
    Human Sin1/MAPKAP1 Antibody MAB8168

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Associated Pathways

mTOR Signaling Pathway mTOR Signaling Pathway Thumbnail