Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SNF1LK2/SIK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SNF1LK2
Ala663-Leu818
Accession # Q9H0K1

Specificity

Detects human SNF1LK2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human SNF1LK2 Antibody

SNF1LK2 antibody in Human Adipocytes by Immunocytochemistry (ICC).

SNF1LK2 in Human Adipocytes.

SNF1LK2 was detected in immersion fixed human adipocytes using 10 µg/mL Goat Anti-Human SNF1LK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5737) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human SNF1LK2 antibody by Western Blot.

Detection of Human SNF1LK2 by Western Blot.

Western blot shows lysates of human mesenchymal stem cells (MSC) grown in the absence (-) or presence (+) of StemXVivo™ Adipogenic Supplement (Catalog # CCM001). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SNF1LK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5737), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SNF1LK2 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human SNF1LK2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human adipocytes

Western Blot

1 µg/mL
Sample: Human mesenchymal stem cells (MSC) cultured with StemXVivo™ Adipogenic Supplement (Catalog # CCM001)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SNF1LK2/SIK2

SNF1LK2 (Sucrose non-fermenting 1 like kinase 2) also known as SIK2 (Salt-inducible kinase 2) is a cytoplasmic 125 kDa member of the CAMK Ser/Thr protein kinase family, AMPK subfamily of enzymes. It is expressed in preadipocytes/adipocytes, with levels strongly induced during early differentiation. SNF1LK2 regulates the early phase of insulin signaling by phosphorylating IRS-1 on Ser794. Human SNF1LK2 is 926 amino acids (aa) in length and contains a protein kinase domain (aa 20‑271) with an ATP binding site (Lys49) and a catalytic loop (aa 140‑149), followed by a phosphorylation activation site at Thr175, a UBA (ubiquitin-associated) domain (aa 295‑335) and another PKA-dependent phosphorylation site at Ser587.

Long Name

SNF1-like Kinase 2

Alternate Names

LOH11CR1I, QIK, SIK2

Entrez Gene IDs

23235 (Human); 235344 (Mouse)

Gene Symbol

SIK2

UniProt

Q9H0K1

Additional SNF1LK2/SIK2 Products

Product Documents for Human SNF1LK2 Antibody

Certificate of Analysis

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Product Specific Notices for Human SNF1LK2 Antibody

For research use only

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Protocols

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