Human SOX15 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF4070X Datasheet / COA / SDS

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AF4070X-100UG

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All SOX15 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX15 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOX15 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) SOX3, rhSOX14, and rhSOX21 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SOX15
Met1-Leu233
Accession # O60248

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SOX15

SOX15 (also known as SOX20) is a nuclear, 25 kDa transcriptional repressor that belongs to group G of the SOX gene family. Human SOX15 is 233 amino acids (aa) in length and contains a 63 aa DNA-binding HMG-box (amino acids 48‑110). Although the C-terminus (aa 126‑233) does not bind DNA, it is required for SOX15 repressive activity. It may accomplish this by interacting with beta -catenin. SOX15 is expressed in myoblasts and regulates skeletal muscle regeneration and differentiation. Human SOX15 shares 75% aa sequence identity with mouse SOX15.

Long Name

Transcription Factor SOX15

Entrez Gene IDs

6665 (Human); 20670 (Mouse); 363632 (Rat)

Alternate Names

protein SOX-15; SOX15; SOX20; SOX20Protein SOX-20; SOX26; SOX26SOX12; SOX27; SOX27Protein SOX-12; SRY (sex determining region Y)-box 15; SRY (sex determining region Y)-box 20