SOX15 (also known as SOX20) is a nuclear, 25 kDa transcriptional repressor that belongs to group G of the SOX gene family. Human SOX15 is 233 amino acids (aa) in length and contains a 63 aa DNA-binding HMG-box (amino acids 48‑110). Although the C-terminus (aa 126‑233) does not bind DNA, it is required for SOX15 repressive activity. It may accomplish this by interacting with beta -catenin. SOX15 is expressed in myoblasts and regulates skeletal muscle regeneration and differentiation. Human SOX15 shares 75% aa sequence identity with mouse SOX15.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human SOX15
Met1-Leu233
Accession # O60248
Met1-Leu233
Accession # O60248
Specificity
Detects human SOX15 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) SOX1, 5, 6, 7, 9, 13, and 17 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human SOX15 Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human SOX15
Sample: Recombinant Human SOX15
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: SOX15
Long Name
Transcription Factor SOX15
Alternate Names
SOX20, SOX26, SOX27
Gene Symbol
SOX15
UniProt
Additional SOX15 Products
Product Documents for Human SOX15 Antibody [Biotin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human SOX15 Antibody [Biotin]
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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