Human SREC-I/SCARF1 Antibody

R&D Systems | Catalog # AF2409

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Cotton Rat

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SREC-I/SCARF1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human SREC‑I/SCARF1
Ser20-Thr421
Accession # Q14162

Specificity

Detects SREC‑I/SCARF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SREC-II is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human SREC-I/SCARF1 Antibody

Detection of SREC-I/SR-F1 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of SREC‑I/SR‑F1 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human SREC-I/SR-F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2409, filled histogram) or control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107).

Applications for Human SREC-I/SCARF1 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.030-0.300 µg/mL of this antibody will block 50% of the binding of Recombinant Human SREC-I Fc Chimera (Catalog # 2409-SR) to immobilized human acetylated low-density lipoproteins (AcLDL).

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human SREC-I/SR-F1 Fc Chimera (Catalog # 2409-SR)

Reviewed Applications

Read 1 review rated 4 using AF2409 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SREC-I/SCARF1

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL (OxLDL) (1‑3). Family members are classified alphabetically. They play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I) and SREC-II are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). The full length cDNA of human SREC-I encodes an 830 amino acid (aa) type I transmembrane protein which contains a 19 aa signal peptide, a 402 aa extracellular region, a 21 aa transmembrane segment, and a 388 aa long cytoplasmic domain. The extracellular region contains ten EGF-like repeats (five of which fit the exact consensus sequence for an EGF-like domain) while the cytoplasmic domain is rich in serine and proline in the N-terminal half, and glycine in the C-terminal segment (5, 6). In addition to the full length form, four SREC-I isoforms exist. Two show insertions of a stop codon in EGF-like domain #8, resulting in mature soluble forms of 323 aa and 318 aa, respectively. A third isoform deletes part of domain #8 plus domains #9 and #10; it continues in-frame to generate a mature transmembrane protein of 725 aa. The last isoform shows only cytoplasmic splicing, with 72 aa substituted for the last 332 aa of the full length form. All three transmembrane forms bind acetylated LDL (6). Native SREC-I is approximately 150 kDa in size, and expressed by endothelial cells, macrophages and fetal neurons (7, 8). In the extracellular region human SREC-I is 76% aa identical to mouse SREC-I. The extracellular regions of human SREC-I and II are 53% aa identical.

References

  1. Horiuchi, S. et al. (2003) Amino Acids 25:283.
  2. Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  5. Adachi, H. et al. (1997) J. Biol. Chem. 272:31217.
  6. Adachi, H. and M. Tsujimoto (2002) J. Biol. Chem. 277:24014.
  7. Shibata, M. et al. (2004) J. Biol. Chem. 279:40084.
  8. Tanura, Y. et al. (2004) J. Biol. Chem. 279:30938.

Long Name

Scavenger Receptor Expressed by Endothelial Cells 1

Alternate Names

SCARF1, SR-F1, SRECI

Entrez Gene IDs

8578 (Human)

Gene Symbol

SCARF1

UniProt

Q14162

Additional SREC-I/SCARF1 Products

Product Documents for Human SREC-I/SCARF1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SREC-I/SCARF1 Antibody

For research use only

Citations for Human SREC-I/SCARF1 Antibody

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  • Human SREC-I/SCARF1 Antibody
    Name: Adam Guess
    Application: Flow Cytometry
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 01/20/2017
    Human SREC-I/SCARF1 Antibody AF2409

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