Human TAZ/WWTR1 Antibody (672027)

R&D Systems | Catalog # MAB7210

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 672027
View all TAZ/WWTR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TAZ/WWTR1
Met267-Leu400
Accession # Q9GZV5

Specificity

Detects human TAZ/WWTR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) YAP or rhYes is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human TAZ/WWTR1 Antibody (672027)

Detection of Human TAZ/WWTR1 antibody by Western Blot.

Detection of Human TAZ/WWTR1 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAZ/WWTR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TAZ/WWTR1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

TAZ/WWTR1 in BG01V Human Embryonic Stem Cells.

TAZ/WWTR1 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

TAZ/WWTR1 in A431 Human Cell Line.

TAZ/WWTR1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human TAZ/WWTR1 Monoclonal Antibody (Catalog # MAB7210) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human TAZ/WWTR1 by Western Blot

Detection of Human TAZ/WWTR1 by Western Blot

Downregulation of CRB3 confers CSC traits on breast cancer cells through the TAZ/ beta -catenin cascade. (a) Western blot detecting the expressions of AMOT, TAZ and beta -catenin. (b) CoIP/western blot analysis showing endogenous AMOT bound to TAZ. (c) Fluorescence-activated cell sorting of CD44high/CD24low population. (d) Western blot of TAZ and stem cell markers. (e, g) Representative images and quantification of formed mammospheres. (f) Western blot of beta -catenin and stem cell markers. XAV939 was used to inhibit beta -catenin. (h) Flow cytometry analysis of the stem cell marker ALDH and the CD44high/CD24low population. All data are presented as mean±s.e.m. and statistical significance was calculated using a two-tailed t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28436991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TAZ/WWTR1 Antibody (672027)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and A431 human epithelial carcinoma cell line

Western Blot

2 µg/mL
Sample: A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB7210 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAZ/WWTR1

TAZ (Transcriptional co-Activatorr with PDZ-binding motif; also WWTR1) is a 50-55 kDa protein that is related to YAP65 TEF-1 interacting protein. It is a widely expressed transcriptional coactivator, and should not be confused with tafazzin/Taz, an enzyme associated with lipid metabolism. TAZ influences the nuclear transport of SMAD-2, -3 and -4, and in the nucleus, TAZ is known to interact with transcription factors such as TFF1, Pax8, NKX2-1 and TEADS, serving as a scaffold for transcriptional activation complexes. Human TAZ is 400 amino acids (aa) in length. It contains one WW domain (aa 124-157) that binds to PPXY motifs, a coiled-coil region (aa 225-259), and a PDZ binding domain (aa 394-400). There are at least four utilized phosphorylation sites. When phosphorylated on Ser89, TAZ preferentially bind to 14-3-3 proteins, promoting its retention in the cytoplasm. Over aa 267-400, human TAZ shares 88% aa identity with mouse TAZ.

Long Name

Transcriptional Coactivator with PDZ-binding Motif

Alternate Names

WWTR1

Entrez Gene IDs

25937 (Human); 97064 (Mouse); 295062 (Rat)

Gene Symbol

WWTR1

UniProt

Q9GZV5 (Human)

Additional TAZ/WWTR1 Products

Product Documents for Human TAZ/WWTR1 Antibody (672027)

Certificate of Analysis

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Product Specific Notices for Human TAZ/WWTR1 Antibody (672027)

For research use only

Citations for Human TAZ/WWTR1 Antibody (672027)

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  • Human TAZ/WWTR1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: BG01V human embryonic stem cells
    Species: Human
    Verified Customer | Posted 12/23/2021
    Human TAZ/WWTR1 Antibody (672027) MAB7210

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