Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 393720
View all TBX2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TBX2
Thr593-Arg702
Accession # Q13207

Specificity

Detects human TBX2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX3, rhTBX6, or rhT‑bet is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human TBX2 Antibody

TBX2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

TBX2 in MCF‑7 Human Cell Line.

TBX2 was detected in immersion fixed MCF-7 human breast cancer cell line using Rat Anti-Human TBX2 Monoclonal Antibody (Catalog # MAB50401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TBX2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TBX2

T-box protein 2 (TBX2) is a member of the T-box family of transcription factors and functions in the transcriptional regulation of genes required for mesoderm differentiation and limb bud pattern formation. The 74 kDa (predicted MW) nuclear protein, like other T-box family members, contains a T-domain which is involved in DNA binding and dimerization of the protein (aa 99‑277). Human TBX2 shows 96%, 95%, and 71% amino acid identity with dog, mouse, and Xenopus tropicalis TBX2, respectively.

Long Name

T-box Transcription Factor 2

Alternate Names

FLJ10169, T-box 2, T-box protein 2, T-box transcription factor TBX2

Entrez Gene IDs

6909 (Human); 21385 (Mouse); 303398 (Rat)

Gene Symbol

TBX2

UniProt

Q13207

Additional TBX2 Products

Product Documents for Human TBX2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TBX2 Antibody

For research use only

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