Human TFEB Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB9170S Datasheet / COA / SDS

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FAB9170S-100UG

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Human TFEB Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFEB in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG2b Clone # 954604

Purification

Protein A or G purified

Immunogen

Human TFEB synthetic peptide
Pro384-Ala446
Accession # P19484

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TFEB

Transcription Factor EB/TFEB binds E-box (5'-CANNTG-3') where it plays a role in T cell activation through expression of CD40L and CLEAR-box (5'-GTCACGTGAC-3') sequences where it promotes expression of lysosomal genes essential for regulation of autophagy. TFEB is localized to the cytoplasm and translocates to the nucleus under aberrant lysosomal storage conditions. Phosphorylation of S211 in an MTORC1 dependent manner sequesters TFEB in the cytoplasm through association with 14-3-3 family proteins. Inhibition of MTORC1 causes dissociation from this complex and rapid accumulation in the nucleus.

Long Name

Transcription Factor EB

Entrez Gene IDs

7942 (Human); 21425 (Mouse); 316214 (Rat)

Alternate Names

ALPHATFEB; BHLHE35; Class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; TCFEBbHLHe35; TFEB; transcription factor EB