Human Thrombospondin-2 Antibody (230934)

R&D Systems | Catalog # MAB1635

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 230934
View all Thrombospondin-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-2
Gly19-Ile1172
Accession # P35442

Specificity

Detects human Thrombospondin-2 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human Thrombospondin‑1, -3, or -4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Thrombospondin-2 Antibody (230934)

Detection of Mouse Thrombospondin-2 by Western Blot

Detection of Mouse Thrombospondin-2 by Western Blot

Endothelial cells and TGF beta are not affected by cardiac Thbs1 overexpression.a Quantification of capillary number per mm2 of tissue from histological sections of tTA cont. and Thbs1 DTG hearts stained with isolectin B4 at 6 weeks of age. b Quantification of endothelial cell proliferation as measured by EdU incorporation co-labeled with CD31 in tTA cont. and Thbs1 DTG hearts at 6 weeks of age. c Quantification of endothelial cell apoptosis detected by TUNEL staining co-labeled with isolectin B4 in tTA cont. and Thbs1 DTG hearts at 6 weeks of age. d ELISA-based quantification of total TGF beta and e active TGF beta in protein extracts from tTA cont. or Thbs1 DTG hearts at 6 weeks of age. f Schematic diagram of WT Thbs1 domain structure and the Thbs1 delta t1 mutant lacking the Thbs1 type-1 repeat domain region. g Representative western blot analysis for Thbs1 from total protein extracts (Total) and extracellular matrix (ECM) extracts from hearts of tTA cont., Thbs1 DTG, and Thbs1 DTG delta t1 mice at 4 weeks of age. Vinculin is presented as cytosolic control. Coomassie stained (Coom.) gel is shown as loading control. h VW/BW ratio at 4 weeks of age in the indicated groups of mice. *P < 0.0001 versus tTA cont.; statistical analysis was performed using one-way ANOVA and Tukey multiple comparisons test. i Kaplan–Meier survival plot of tTA cont., Thbs1 DTG, and Thbs1 delta t1 DTG animals. *P < 0.0001 vs tTA cont. #P < 0.0001 vs Thbs1 DTG; both analyzed by two-tailed log-rank test. The same data from Fig. 2e are shown again here for tTA cont. and Thbs1 DTG mice (same strain and ages and sex ratio mix). j Representative western blots for Thbs2 and Gapdh as loading control, from heart protein extracts from tTA cont. and Thbs2 DTG mice at 8 weeks of age. k Heart weight (HW)/BW ratio, and l FS percentage at 8 weeks of age from tTA cont. and Thbs2 DTG mice. The number of biologically independent animals analyzed is indicated on each graph. Error bars are ±standard error of the mean. Source data are prov

Applications for Human Thrombospondin-2 Antibody (230934)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Thrombospondin‑2 (Catalog # 1635-T2)

Reviewed Applications

Read 1 review rated 4 using MAB1635 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-2

Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein that modulates cellular interactions with extracellular matrix. Thrombospondin-1 and -2 constitute subgroup A thrombospondin family members and form disulfide-linked homotrimers, whereas Thrombospondin-3, -4, and -5/COMP constitute subgroup B and form homopentamers (1-4). The human TSP-2 cDNA encodes a 1172 amino acid (aa) precursor that includes an 18 aa signal sequence followed by an N-terminal
heparin-binding domain, an oligomerization motif, one vWF-C domain, three TSP type-1 repeats, three EGF-like repeats, seven TSP type-3 repeats, and a lectin-like TSP C‑terminal domain (5). Human TSP-2 shares 88-90% aa sequence identity with bovine, mouse, and rat TSP-2. Within the TSP type-3 repeats and TSP C‑terminal domain, human TSP-2 shares 80% aa sequence identity with human TSP-1 and approximately 60% aa sequence identity with human TSP-3, -4, and -5/COMP. TSP-2 regulates collagen matrix formation by altering fibroblast behavior during development and in areas of tissue remodeling in the adult (6, 7). Trimerization of TSP-2 is required for the calcium-dependent cell attachment and spreading functions, while the heparin-binding domain is responsible for the destabilization of focal adhesion sites (8-10). The heparin-binding domain also mediates binding to Integrins alpha 3 beta 1 and alpha 6 beta 1 on microvascular endothelial cells (EC) and Integrin alpha 4 beta 1 on large blood vessel EC (11, 12). A fragment of TSP-2 (heparin-binding domain, oligomerization motif, and vWF-C domain) promotes EC survival, proliferation, and chemotaxis (11). Inclusion of the three TSP type-1 domains results in a molecule that inhibits VEGF-induced EC migration and vascular tube formation (13, 14). In vivo, full length TSP-2 blocks tumor angiogenesis and induces vascular EC apoptosis (13, 15). HPRG functions as an apparent decoy receptor by preventing interaction of TSP-2 with CD36 on macrophages and microvasculature EC (14). TSP-2 also binds MMP-2 and facilitates MMP-2 clearance by the scavenger receptor LRP (16).

References

  1. Elzie, C.A. and J.E. Murphy-Ullrich (2004) Int. J. Biochem. Cell Biol. 36:1090.
  2. Armstrong, L.C. and P. Bornstein (2003) Matrix Biol. 22:63.
  3. Murphy-Ullrich, J.E. (2001) J. Clin. Invest. 107:785.
  4. Bornstein, P. and E.H. Sage (2002) Curr. Opin. Cell Biol. 14:608.
  5. LaBell, T.L. and P.H. Byers (1993) Genomics 17:225.
  6. Kyriakides, T.R. et al. (1998) J. Histochem. Cytochem. 46:1007.
  7. Kyriakides, T.R. et al. (1998) J. Cell Biol. 140:419.
  8. Anilkumar, N. et al. (2002) J. Cell Sci. 115:2357.
  9. Misenheimer, T.M. et al. (2003) Biochemistry 42:5125.
  10. Murphy-Ullrich, J.E. et al. (1993) J. Biol. Chem. 268:26784.
  11. Calzada, M.J. et al. (2004) Circ. Res. 94:462.
  12. Calzada, M.J. et al. (2003) J. Biol. Chem. 278:40679.
  13. Noh, Y-H. et al. (2003) J. Invest. Dermatol. 121:1536.
  14. Simantov, R. et al. (2005) Matrix Biol. 24:27.
  15. Streit, M. et al. (1999) Proc. Natl. Acad. Sci. 96:14888.
  16. Yang, Z. et al. (2001) J. Biol. Chem. 276:8403.

Alternate Names

THBS2, Thrombospondin2, TSP-2

Entrez Gene IDs

7058 (Human)

Gene Symbol

THBS2

UniProt

P35442

Additional Thrombospondin-2 Products

Product Documents for Human Thrombospondin-2 Antibody (230934)

Certificate of Analysis

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Product Specific Notices for Human Thrombospondin-2 Antibody (230934)

For research use only

Citations for Human Thrombospondin-2 Antibody (230934)

Customer Reviews for Human Thrombospondin-2 Antibody (230934) (1)

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  • Human Thrombospondin-2 Antibody
    Name: Lucas Tomko
    Application: Western Blot
    Sample Tested: Heparin Plasma
    Species: Human
    Verified Customer | Posted 06/30/2016
    Lanes in the WB are alternating between normal and diseased patients. Bands for thrombospondin-2 were resolved at the predicted MW; however, there was no difference between patients in this experiment. The results were clean, though, so I would consider using this for other applications in the future.
    Human Thrombospondin-2 Antibody (230934) MAB1635

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Associated Pathways

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