Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1009524
View all TMED1 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293 derived human TMED1
Met1-Asn194
Accession # Q13445

Specificity

Detects human TMED1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TMED1 Antibody

Detection of Human TMED1 antibody by Western Blot.

Detection of Human TMED1 by Western Blot.

Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line, HDLM-2 human Hodgkin's lymphoma cell line, RT-4 human bladder carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TMED1 Monoclonal Antibody (Catalog # MAB22431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TMED1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of TMED1 antibody in HEK293 Human Cell Line Transfected with Human TMED1 and eGFP antibody by Flow Cytometry.

Detection of TMED1 in HEK293 Human Cell Line Transfected with Human TMED1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human TMED1 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human TMED1 Monoclonal Antibody (Catalog # MAB22431) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TMED1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human TMED1 and eGFP

Western Blot

1 µg/mL
Sample: U‑87 MG human glioblastoma/astrocytoma cell line, HDLM‑2 human Hodgkin's lymphoma cell line, RT-4 human bladder carcinoma cell line, and PC‑3 human prostate cancer cell line

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMED1

TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking (1, 2). TMED1 is a 59 kDa monomer and has been reported to exist as homodimer (3). TMED1 is composed of a 23 amino acid (aa) signal sequence, a 171 aa extra cellular domain, a 21 aa transmembrane domain, and a 12 aa cytoplasmic domain. The extracellular domain contains an 83 aa GOLD (Golgi Dynamics) domain, and COPI and COPII binding motifs are found in the cytoplasmic domain (1-3, 5). Human TMED1 shares 97% sequence identity with mouse, bovine, and rat homologs within the 171 aa extracellular domain. The beta -strand-rich GOLD domain has been specifically identified to be involved in intracellular protein trafficking (1, 4, 5). TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL‑33 (1). This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK (1, 6). Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions (1).

References

  1. Connolly, D. et al. (2013) J Biol Chem. 288:5616.
  2. Gour, N. and Lajoie, S. (2018) Curr Allergy Ashma Rep. 16:65.
  3. Jenne, N. (2002) J Biol Chem. 277:46504.
  4. Anantharaman, V. and Aravind, L. (2002) Genome Biol. 3:research0023
  5. Gomez-Navarro, N. and Miller, E. (2016) J Cell Biol. 215:769.
  6. Hardman, C. and Ogg, G. (2016). Curr Opin Immunol. 42:16.

Alternate Names

IL-1RL1LG, IL1RL1-Binding Protein, Il1rl1l, IL1RL1LG, Interleukin 1 Receptor-Like 1 Ligand, Ly84l, P24g1, P24gamma1, Tp24

Entrez Gene IDs

11018 (Human); 17083 (Mouse); 315461 (Rat)

Gene Symbol

TMED1

UniProt

Q13445

Additional TMED1 Products

Product Documents for Human TMED1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TMED1 Antibody

For research use only

Related Research Areas

Group 2 ILCs
IL-1 Family

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