Human TOP2A Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB6540T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB6540T-100UG

Save 15% on Select RUO Reagents. See Details.

All TOP2A Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TOP2A Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TOP2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant TOP2B from human or mouse is observed.

Source

Monoclonal Mouse IgG2a Clone # 680130

Purification

Protein A or G purified

Immunogen

E.coli-derived recombinant human TOP2A
Phe1174-Phe1531
Accession # P11388

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TOP2A

DNA topoisomerase 2-alpha (TOP2A; also DNA topoisomerase II, alpha isozyme) is a 170 kDa member of the type II topoisomerase family. Human TOP2A is 1531 amino acids (aa) in length, and splicing variants produce three additional isoforms which have substitutions following Lys321, Gln355, or Ala401. TOP2A contains a histidine kinase-like ATPases domain (aa 80-193), a TopoIIA Trans ScTopoIIA domain (aa 265-417), a TOPRIM TopoIIA: topoisomerase-primase domain (aa 455-575), a DNA Topoisomerase IV domain (aa 697-1169), and a NES (aa 1018-1028). There are multiple potential Ser/Thr phosphorylation sites. Human TOP2A shares 89% aa sequence identity with mouse TOP2A.

Long Name

DNA Topoisomerase II alpha

Entrez Gene IDs

7153 (Human)

Alternate Names

DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; TOP2; TOP2A; TOP2DNA gyrase; topoisomerase (DNA) II alpha (170kD); topoisomerase (DNA) II alpha 170kDa; TP2A