Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 680130
View all TOP2A Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human TOP2A
Phe1174-Phe1531
Accession # P11388

Specificity

Detects human TOP2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant TOP2B from human or mouse is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TOP2A Antibody

Detection of Human TOP2A antibody by Western Blot.

Detection of Human TOP2A by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human TOP2A Monoclonal Antibody (Catalog # MAB6540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TOP2A at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TOP2A antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

TOP2A in HeLa Human Cell Line.

TOP2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human TOP2A Monoclonal Antibody (Catalog # MAB6540) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TOP2A Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TOP2A

DNA topoisomerase 2-alpha (TOP2A; also DNA topoisomerase II, alpha isozyme) is a 170 kDa member of the type II topoisomerase family. Human TOP2A is 1531 amino acids (aa) in length, and splicing variants produce three additional isoforms which have substitutions following Lys321, Gln355, or Ala401. TOP2A contains a histidine kinase-like ATPases domain (aa 80-193), a TopoIIA Trans ScTopoIIA domain (aa 265-417), a TOPRIM TopoIIA: topoisomerase-primase domain (aa 455-575), a DNA Topoisomerase IV domain (aa 697-1169), and a NES (aa 1018-1028). There are multiple potential Ser/Thr phosphorylation sites. Human TOP2A shares 89% aa sequence identity with mouse TOP2A.

Long Name

DNA Topoisomerase II alpha

Alternate Names

TOP2, TP2A

Entrez Gene IDs

7153 (Human)

Gene Symbol

TOP2A

UniProt

P11388

Additional TOP2A Products

Product Documents for Human TOP2A Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TOP2A Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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Protocols

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