Human TrkB Alexa Fluor® 647-conjugated Antibody
Human TrkB Alexa Fluor® 647-conjugated Antibody Summary
Accession # Q16620
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TrkB in Human TrkB transfected C6 Cell Line by Flow Cytometry. C6 cell line transfected with human TrkB was stained with Mouse Anti-Human TrkB Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB3971R, filled histogram) or isotype control antibody (IC002R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TrkB in Baf3 cells by Flow Cytometry. Baf3 cells transfected with Human TrkB (filled histogram) vs Irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human TrkB Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB3971R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and
NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.
- Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
- Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
- Soppet, D. et al. (1991) Cell 65:895.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human TrkB Alexa Fluor® 647-conjugated Antibody
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ERBB3 overexpression due to miR-205 inactivation confers sensitivity to FGF, metabolic activation, and liability to ERBB3 targeting in glioblastoma
Authors: F De Bacco, F Orzan, J Erriquez, E Casanova, L Barault, R Albano, A D'Ambrosio, V Bigatto, G Reato, M Patanè, B Pollo, G Kuesters, C Dell'Aglio, L Casorzo, S Pellegatta, G Finocchiar, PM Comoglio, C Boccaccio
Cell Reports, 2021-07-27;36(4):109455.
Sample Types: Whole Cells
Applications: Flow Cytometry
Peripheral blood brain-derived neurotrophic factor level and tyrosine kinase B expression on T lymphocytes in systemic lupus erythematosus: Implications for systemic involvement
Authors: B Tian, C Yang, J Wang, X Hou, S Zhao, Y Li, P Yang
Sample Types: Whole Cells
Applications: Flow Cytometry
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