Human TROY/TNFRSF19 Antibody

Catalog #: MAB15481 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB15481-100
MAB15481-SP

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Detection of TROY/TNFRSF19 in HEK293 Human Cell Line Transfected with Human TROY/TNFRSF19 by Flow Cytometry.

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Human TROY/TNFRSF19 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TROY/TNFRSF19 in direct ELISAs. Stains human TROY/TNFRSF19 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 933202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TROY/TNFRSF19
Glu30-Leu170
Accession # Q9NS68

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TROY/TNFRSF19 in HEK293 Human Cell Line Transfected with Human TROY/TNFRSF19 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either human TROY/TNFRSF19 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human TROY/ TNFRSF19 Monoclonal Antibody (Catalog # MAB15481), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROY/TNFRSF19

TROY (also known as TAJ and TNFRSF19) is a 45‑55 kDa member of the TNF receptor superfamily of molecules (1‑4). It is widely expressed in the embryo, but shows limited expression in adult. In particular, embryonic TROY is found in embryonic epithelium, neural tube, and mesenchyme, and developing neurons of the hippocampus and thalamus (5, 6). In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles where it is expressed by dermal papilla matrix cells (4, 7, 8, 9). In human, TROY has also been found on melanoma cells, not normal melanocytes (10). TROY is reportedly a component of the Nogo-A receptor, taking the place of p75 in a membrane-bound LINGO-1:NgR1:TROY complex (6, 8, 9). This complex purportedly mediates axon repulsion initiated by one of five myelin-associated inhibitory factors (OMpg; CD100; Ephrin B3; Nogo-A; MAG) (6, 11). In the hair follicle, TROY binds LTa and acts with Eda to drive secondary hair follicle development (4, 12). Mature human TROY is a 394 amino acid (aa) type I transmembrane protein (1‑3). It possesses a 141 aa extracellular region (aa 30‑170) that contains three 40 aa TNFR cysteine motifs, and an extended 232 aa cytoplasmic domain (aa 192‑423). There is one potential isoform that shows a two aa substitution for aa 416‑423. The extracellular domains of human and mouse TROY share 90% aa identity.

References

Hu, S. et al. (1999) Genomics 62:103.
Eby, M.T. et al. (2000) J. Biol. Chem. 275:15336.
Kojima, T. et al. (2000) J. Biol. Chem. 275:20742.
Hashimoto, T. et al. (2008) Cell Cycle 7:106.
Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
Mi, S. (2008) Cytokine Growth Factor Rev. 19:245.
Satoh, J. et al. (2007) Neuropathol. Appl. Neurobiol. 33:99.
Shao, Z. et al. (2005) Neuron 45:353.
Park, J.B. et al. (2000) Neuron 45:345.
Spanjaard, R.A. et al. (2007) Int. J. Cancer 120:1304.
Venkatesh, K. et al. (2007) J. Cell Biol. 177:393.
Pispa, J. et al. (2008) Hum. Mol. Genet. 17:3380.

Long Name

Tumor Necrosis Factor Receptor Superfamily, Member 19

Entrez Gene IDs

55504 (Human); 29820 (Mouse)

Alternate Names

TAJ; TAJ-alpha; TAJToxicity and JNK inducer; TNFRSF19; TRADE; TROY; TROYtumor necrosis factor receptor superfamily member 19; tumor necrosis factor receptor superfamily, member 19