Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Xenopus

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 549115
View all VAMP-7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VAMP‑7
Ala2-Met140
Accession # P51809

Specificity

Detects human VAMP‑7 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human VAMP‑7 Antibody

Detection of Human VAMP-7 antibody by Western Blot.

Detection of Human VAMP‑7 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human VAMP-7 Monoclonal Antibody (Catalog # MAB6117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human VAMP-7 by Western Blot

Detection of Human VAMP-7 by Western Blot

Amphisome/lysosome fusion is not required for autophagy-mediated exosomal secretion of ANXA2. (a) Cells with VAMP7 knockdown and control knockdown were transfected with a GFP-LC3 plasmid and treated with or without 500 U/ml IFN-gamma for 24 h. Cells were then fixed, permeabilized, and stained for ANXA2 (red) and LAMP1 (blue). The colocalization of ANXA2, GFP-LC3 and LAMP1 was observed by confocal microscopy. Scale bar: 10 μm. The arrow and dotted inset mark an autolysosome. (b) Line tracing analysis of fluorescence signal from image in (a) of VAMP7 knockdown and control knockdown cells after IFN-gamma stimulation is shown. (c) VAMP7 knockdown efficiency was detected by western blotting. Control and VAMP7-silenced cells were treated with or without 500 U/ml IFN-gamma for 48 h. The exosome pellets were collected. ANXA2, alpha -tubulin, Tsg101 and calnexin from exosome pellets and total cell lysates were detected by western blotting. kD, molecular weight as kDa. (d) A549 cells were incubated with 500 U/ml IFN-gamma in the presence or absence of 5 nM bafilomycin A1 for 48 h. ANXA2 and alpha -tubulin from cultured supernatant and total cell lysate were analyzed by western blotting. kD, molecular weight as kDa. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28720835), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human VAMP‑7 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human breast and human breast cancer tissue

Western Blot

1 µg/mL
Sample: A172 human glioblastoma cell line and K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB6117 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAMP-7

Vesicle-associated membrane protein 7 (VAMP-7) is a 25 kDa, widely expressed, type IV transmembrane protein and member of the synaptobrevin family. Mature human VAMP-7 consists of a 187 aa cytoplasmic domain, a 21 aa transmembrane region, and an 11 aa vesicular region. The cytoplasmic domain contains a longin domain (aa 7‑110) and a v-SNARE coiled-coil homology domain (aa 125‑185). Two splicing variants produce three isoforms for human VAMP-7. Isoform 2 has a 116 aa substitution for aa 145‑220 found in isoform 1, and isoform 3 is missing the residues corresponding to aa 28‑68 in isoform 1. Human VAMP-7 shares 99%, 97%, and 95% aa sequence identity with bovine, mouse, and rat VAMP-7, respectively.

Long Name

Vesicle-Associated Membrane Protein 7

Alternate Names

SYBL1, TI-VAMP, VAMP7

Entrez Gene IDs

6845 (Human); 20955 (Mouse); 85491 (Rat)

Gene Symbol

VAMP7

UniProt

P51809 (Human)

Additional VAMP-7 Products

Product Documents for Human VAMP‑7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VAMP‑7 Antibody

For research use only

Related Research Areas

Neuronal Lineage Markers

Citations for Human VAMP‑7 Antibody

Customer Reviews for Human VAMP‑7 Antibody (2)

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Customer Images

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  • Human VAMP-7 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 11/27/2024
    Used for intracellular staining with Flow Cytometry/CyTOF
  • Human VAMP-7 Antibody
    Name: Albert Tsai
    Application: CyTOF
    Sample Tested: bone marrow and Peripheral blood
    Species: Human
    Verified Customer | Posted 03/18/2020
    VAMP-7 by mass cytometry recapitulates side scatter by flow cytometry. Nat Med. 2020. 26:408-417.
    Human VAMP‑7 Antibody MAB6117

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