Human VEGF-C Antibody (193208)

R&D Systems | Catalog # MAB752

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 193208
View all VEGF-C Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VEGF-C
Thr103-Arg227 (Cys156Ser)
Accession # Q6FH59

Specificity

Detects human VEGF-C in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) PDGF‑AA, rhPDGF‑BB, rhPlGF, rhVEGF

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human VEGF-C Antibody (193208)

Human VEGF‑C ELISA Standard Curve.

Recombinant Human VEGF‑C protein was serially diluted 2-fold and captured by Mouse Anti-Human VEGF‑C Monoclonal Antibody (Catalog # MAB752) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (AF752) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human VEGF-C Antibody (193208)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human VEGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF752).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human VEGF-C DuoSet ELISA Kit (Catalog # DY752B) for convenient development of a sandwich ELISA or the Human VEGF-C Quantikine ELISA Kit (Catalog # DVEC00) for  a complete optimized ELISA.

Western Blot

1 µg/mL
Sample: Recombinant Human VEGF-C (Catalog # 2179-VC)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF-C

Vascular Endothelial Growth Factor C (VEGF-C) is a member of the VEGF family of secreted growth factors. The VHD form homodimers after proteolytic removal of the N- and C-terminal pro regions. VEGF-C promotes angiogenesis and lymphangiogenesis through interactions with VEGF R2 and VEGF R3.

Long Name

Vascular Endothelial Growth Factor C

Alternate Names

VEGFC

Entrez Gene IDs

7424 (Human); 22341 (Mouse)

Gene Symbol

VEGFC

UniProt

Q6FH59

Additional VEGF-C Products

Product Documents for Human VEGF-C Antibody (193208)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VEGF-C Antibody (193208)

For research use only

Citations for Human VEGF-C Antibody (193208)

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