Human VEGFR1/Flt-1 DuoSet ELISA

Name: Human VEGFR1/Flt-1 DuoSet ELISA
Brand: R&D Systems
SKU: DY321B
Rating: 5 (2 reviews)

DY321B 17 Citations 2 Reviews Datasheet / COA / SDS

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DY321B

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DY008: DuoSet ELISA Ancillary Reagent Kit 2

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Human VEGF R1 / Flt-1 ELISA Standard Curve

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Product Details

Citations (17)

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Reviews (2)

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human VEGFR1/Flt-1 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VEGF R1 / Flt-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

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Human VEGF R1 / Flt-1 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR1/Flt-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Long Name:

Vascular Endothelial Growth Factor Receptor 1

Entrez Gene IDs:

2321 (Human); 14254 (Mouse)

Alternate Names:

EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

Citations for Human VEGFR1/Flt-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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No association between early antiretroviral therapy during pregnancy and plasma levels of angiogenic factors: a cohort study
Authors: A Djeha, S Girard, H Trottier, F Kakkar, H Soudeyns, M Boucher, N Lapointe, I Boucoiran
BMC Pregnancy Childbirth, 2019;19(1):482.
Species: Human
Sample Types: Serum
Sulfasalazine reduces placental secretion of antiangiogenic factors, up-regulates the secretion of placental growth factor and rescues endothelial dysfunction
Authors: FC Brownfoot, NJ Hannan, P Cannon, V Nguyen, R Hastie, LJ Parry, S Senadheera, L Tuohey, S Tong, TJ Kaitu'u-Li
EBioMedicine, 2019;0(0):.
Species: Human
Sample Types: Tissue Culture Supernates
Combinations of Bevacizumab and Erlotinib show activity in colorectal cancer independent of RAS status
Authors: P Mésange, A Bouygues, N Ferrand, M Sabbah, AE Escargueil, A Savina, B Chibaudel, C Tournigand, T Andre, A de Gramont, AK Larsen
Clin. Cancer Res., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Melatonin enhances antioxidant molecules in the placenta, reduces secretion of soluble fms-like tyrosine kinase 1 (sFLT) from primary trophoblast but does not rescue endothelial dysfunction: An evaluation of its potential to treat preeclampsia
Authors: NJ Hannan, NK Binder, S Beard, TV Nguyen, TJ Kaitu'u-Li, S Tong
PLoS ONE, 2018;13(4):e0187082.
Species: Human
Sample Types: Cell Culture Supernates
Placental Dysfunction Underlies Increased Risk of Fetal Growth Restriction and Stillbirth in Advanced Maternal Age Women
Authors: SC Lean, AEP Heazell, MR Dilworth, TA Mills, RL Jones
Sci Rep, 2017;7(1):9677.
Species: Human
Sample Types: Serum
Tuberculosis-diabetes co-morbidity is characterized by heightened systemic levels of circulating angiogenic factors.
Authors: Kumar N, Moideen K, Sivakumar S, Menon P, Viswanathan V, Kornfeld H, Babu S
J Infect, 2017;74(1):10-21.
Species: Human
Sample Types: Plasma
Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
Oncol Lett, 2017;13(5):3269-3276.
Species: Human
Sample Types: Cell Culture Supernates
Circulating Angiogenic Factors as Biomarkers of Disease Severity and Bacterial Burden in Pulmonary Tuberculosis.
Authors: Kumar N, Banurekha V, Nair D, Babu S
PLoS ONE, 2016;11(1):e0146318.
Species: Human
Sample Types: Plasma
Angiogenic proteins, placental weight and perinatal outcomes among pregnant women in Tanzania
PLoS ONE, 2016;11(12):e0167716.
Species: Human
Sample Types: Plasma
Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
PLoS ONE, 2015;10(8):e0134619.
Species: Human
Sample Types: Plasma

Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
BMC Infect Dis, 2014;14(0):35.
Species: Human
Sample Types: Serum

Serum concentrations of soluble Flt-1 are decreased among women with a viable fetus and no symptoms of miscarriage destined for pregnancy loss.
Authors: Kaitu'u-Lino TJ, Whitehead CL, Ngian GL, Permezel M, Tong S
PLoS ONE, 2012;7(2):e32509.
Species: Human
Sample Types: Serum

Differential expression of Vegfr-2 and its soluble form in preeclampsia.
Authors: Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM
PLoS ONE, 2012;7(3):e33475.
Species: Human
Sample Types: Plasma

Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
PLoS ONE, 2011;6(2):e17440.
Species: Human
Sample Types: Plasma

Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
Authors: Conroy AL, Liles WC, Molyneux ME
PLoS ONE, 2011;6(12):e28540.
Species: Human
Sample Types: Plasma

Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
Placenta, 2010;31(6):545-8.
Species: Human
Sample Types: Cell Culture Supernates

Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
J. Immunol., 2009;183(12):8176-85.
Species: Human
Sample Types: Cell Culture Supernates