Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1097A
View all XCR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094

Specificity

Detects human XCR1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human XCR1 PE‑conjugated Antibody

Detection of XCR1 antibody in CD141+HLA-DR+Human Blood Monocytes antibody by Flow Cytometry.

Detection of XCR1 in CD141+HLA-DR+Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with (A) Mouse Anti-Human HLA-DR Fluorescein-Conjugated Monoclonal Antibody (Catalog # FAB4869F), Mouse Anti-Human Thrombomodulin/BDCA-3 (CD141) APC-Conjugated Monoclonal Antibody (Catalog # FAB3947A) and (B) Rabbit Anti-Human XCR1 PE-conjugated Monoclonal Antibody (Catalog # FAB8571P, filled histogram) or isotype control antibody (Catalog # IC105P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human XCR1 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CD141+ HLA-DR+ human peripheral blood monocytes

Reviewed Applications

Read 1 review rated 5 using FAB8571P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: XCR1

XCR1, also known as GPR5 and Lymphotactin/SCM-1 (Single Cysteine Motif 1) Receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/Lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, Human Herpes Virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells, NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

Long Name

Chemokine [C Motif] Receptor 1

Alternate Names

CCXCR1, GPR5

Entrez Gene IDs

2829 (Human); 23832 (Mouse); 301086 (Rat)

Gene Symbol

XCR1

UniProt

P46094

Additional XCR1 Products

Product Documents for Human XCR1 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human XCR1 PE‑conjugated Antibody

For research use only

Citations for Human XCR1 PE‑conjugated Antibody

Customer Reviews for Human XCR1 PE‑conjugated Antibody (1)

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  • Human XCR1 PE-conjugated Antibody
    Name: ming sun
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 06/24/2018
    this antibody was used to stain BDCA3 defined by XCR1+BDCA3+ dendritic cells and it works very well.

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