ICAM-1/CD54 Antibody (166623) [PerCP]

Name: ICAM-1/CD54 Antibody (166623) [PerCP]
Brand: Novus Biologicals
SKU: FAB796C
Price: 569 USD
Availability: InStock

Novus Biologicals | Catalog # FAB796C

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Applications

Western Blot, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair)

Label

PerCP (Excitation = 490 nm, Emission = 675 nm)

Antibody Source

Monoclonal Rat IgG_2B Clone # 166623

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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1
Gln28-Asn485
Accession # P13597

Specificity

Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and rmICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, rrICAM-1, rmMAdCAM-1, or rmVCAM-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for ICAM-1/CD54 Antibody (166623) [PerCP]

Application

Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Recommended applications are based on validated applications from the unconjugated base product MAB796. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (166623) [PerCP]

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Certificate of Analysis

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Product Specific Notices for ICAM-1/CD54 Antibody (166623) [PerCP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.