Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence

Label

DyLight 680 (Excitation = 692 nm, Emission = 712 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Fc Secondary Antibodies »

Product Specifications

Immunogen

Goat anti-Mouse IgG Fc fragment Secondary Antibody was produced by repeated immunization with Mouse IgG F(c) fragment in goat.

Specificity

No reaction was observed against Mouse IgG F(ab) This antibody will react with heavy chains of Mouse IgG. Minimal reactivity is expected against other Mouse immunoglobulins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum

Scientific Data Images for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680]

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680] [NBP1-72880] - The cell line was stained with 488 (green) and 680 (red) secondary antibodies for 30min at RT. Image from verified customer review.
Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680] [NBP1-72880] - Fluorescence absorption and emission spectra of Dylight 680 in PBS, pH 7.2.
Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680]

Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680]

DyLight(TM) dyes can be used for two-color Western Blot detection with low background and high signal. Anti-tubulin was detected using a DyLight(TM) 680 conjugate. Anti-TNFa was detected using a DyLight(TM) 800 conjugate. The image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR.

Applications for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680]

Application
Recommended Usage

Fluorophore-linked immunosorbent assay

1:20000

Immunocytochemistry/ Immunofluorescence

1:5000

Western Blot

1:10000
Application Notes
This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-72880 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Product Documents for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680]

Customer Reviews for Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680] (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

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  • Goat anti-Mouse IgG Fc fragment Secondary Antibody [DyLight 680]
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human breast cancer cell line SKBR3 and this product
    Species: Human
    Verified Customer | Posted 02/27/2019
    The cell line was stained with 488 (green) and 680 (red) secondary antibodies for 30min at RT
    Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680] NBP1-72880
  • Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680] (Pre-adsorbed)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: breast cancer MCF7 cells
    Species: Human
    Verified Customer | Posted 06/28/2018
    Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 680] NBP1-72880

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Protocols

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FAQs

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