Rat IgG2a Isotype Control (2A3)

Novus Biologicals | Catalog # NBP2-21947

Novus Biologicals
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Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 2A3
View all IgG2A Isotype Controls »

Product Specifications

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

This Rat IgG2A (2A3) immunoglobulin is useful as an isotype control for Rat IgG2A (2A3) antibody. This antibody is purified from culture supernatant of a hybridoma (Mouse myeloma fused with spleen cells from a Rat, clone 2A3) with protein A/G.

Scientific Data Images for Rat IgG2a Isotype Control (2A3)

Flow Cytometry: Rat IgG2a Isotype Control (2A3) [NBP2-21947]

Flow Cytometry: Rat IgG2a Isotype Control (2A3) [NBP2-21947]

Flow Cytometry: Rat IgG2a Isotype Control (2A3) [NBP2-21947] - Flow Cytometry: Rat IgG2a Isotype Control (2A3) [Alexa Fluor® 488] [NBP2-30137] - This image was submitted via customer review. Image using the Alexa Fluor 488 form of this antibody.
Flow Cytometry: Rat IgG2a Isotype Control (2A3) [NBP2-21947]

Flow Cytometry: Rat IgG2a Isotype Control (2A3) [NBP2-21947]

Flow Cytometry: Rat IgG2a Isotype Control (2A3) [NBP2-21947] - Flow analysis of CD45R in 10^6 mouse splenocytes using 0.25 ug of this antibody. The shaded histogram represents mouse splenocytes alone; green represents rat isotype control (20211AF488); red represents this antibody.

Applications for Rat IgG2a Isotype Control (2A3)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with PBS.

Formulation

PBS

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Rat IgG2a Isotype Control (2A3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Rat IgG2a Isotype Control (2A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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