Mouse IgG2a Isotype Control (C1.18.4)

Novus Biologicals | Catalog # NBP2-14864

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # C1.18.4
View all IgG2A Isotype Controls »

Product Specifications

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This Mouse IgG2A (C1.18.4) immunoglobulin is useful as an isotype control for IgG2A (C1.18.4) antibody. The C1.18.4 hybridoma cell line was grown and tissue culture supernatant was purified using Protein G.

Endotoxin Level

<2.0 EU/mg as determined using an LAL gel clotting test.

Scientific Data Images for Mouse IgG2a Isotype Control (C1.18.4)

Flow Cytometry: Mouse IgG2a Isotype Control (C1.18.4) [NBP2-14864]

Flow Cytometry: Mouse IgG2a Isotype Control (C1.18.4) [NBP2-14864]

Flow Cytometry: Mouse IgG2a Isotype Control (C1.18.4) [NBP2-14864] - Cell surface flow analysis of HLA-DR in human lymphocytes using at 1 ug/10^6 cells. Cells were stained with primary antibody followed by a PE-conjugated goat anti-mouse secondary antibody. Green represents isotype control (NBP2-14864); red represents anti-HLA-DR antibody.

Applications for Mouse IgG2a Isotype Control (C1.18.4)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

0.05% BSA, PBS, pH 7

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG2A

A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Mouse IgG2a Isotype Control (C1.18.4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mouse IgG2a Isotype Control (C1.18.4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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