Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # eB121-15F9
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Product Specifications

Immunogen

Mouse IgM

Reactivity Notes

This antibody is reactive to Mouse.

Specificity

This secondary antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugate can be used as a detection secondary for mouse IgM.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [PE]

Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [PE] [NBP1-43749] - See notes for image caption

Applications for Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [PE]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [PE]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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