Q: Does viral IL-10 cross-react in bioassay?

A: In an MC9 bioassay, recombinant EBV IL-10 is 20 times less active than recombinant human IL-10.

Rat IL-10 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-92701

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Citations (2)

Product Documents

Discontinued Product

NBP1-92701 has been discontinued. View all IL-10 products.

Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

1.5 pg/mL

Assay Range

15.6 - 1,000 pg/mL

View all IL-10 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-10 positive serum. There was no crossreactivity detected.

Description

This ELISA kit has been transitioned to a new version, available here: R1000

精度

Intra-Assay Precision (Precision within an assay) CV% 5.0%

Inter-Assay Precision (Precision between assays) CV% 10%

Scientific Data Images for Rat IL-10 ELISA Kit (Colorimetric)

ELISA: Rat IL-10 ELISA Kit (Colorimetric) [NBP1-92701]

ELISA: IL-10 ELISA Kit [Biotin] [NBP1-92701] - Standard curve

Kit Contents for Rat IL-10 ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-10
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Biotin-Conjugate anti-rat IL-10 monoclonal antibody
Rat IL-10 Standard lyophilized, 2 ng/ml upon reconstitution
Sample Diluent
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-10

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The murine IL-10 exhibits strong DNA and aminoacid sequence homology to the human IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for Rat IL-10 ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual - Rat IL-10 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat IL-10 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.