• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Specificity
    Natural and recombinant rat IL-10
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Rat IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-10 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-10 accurately. Results obtained using natural rat IL-10 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-10.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean138270660132270682
Standard Deviation6.3916.113.119.759.8
CV%4.63.32.49.97.38.8

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean103218582114236598
Standard Deviation5.79.517.210.816.442.6
CV%5.54.439.56.97.1

Recovery

The recovery of rat IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 106 96-118
EDTA Plasma (n=6) 100 88-115
Serum (n=6) 94 82-112
Linearity
To assess the linearity of the assay, five or more samples spiked with various concentrations of rat IL-10 in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
Rat IL-10 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-10
Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
    • Long Name
      Interleukin 10
    • Entrez Gene IDs
      3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral);
    • Alternate Names
      CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    27 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in�vitro
      Authors: WP Zheng, BY Zhang, ZY Shen, ML Yin, Y Cao, HL Song
      Mol Med Rep, 2017;15(5):2551-2559.
      Species: Rat
      Sample Type: Cell Culture Supernates
    2. Short-term treatment with taurolidine is associated with liver injury
      Authors: R Fahrner, A Möller, AT Press, A Kortgen, M Kiehntopf, F Rauchfuss, U Settmacher, AS Mosig
      BMC Pharmacol Toxicol, 2017;18(1):61.
      Species: Rat
      Sample Type: Serum
    3. Gene expression profiling and inhibition of adipose tissue accumulation of G. bimaculatus extract in rats on high fat diet.
      Authors: Ahn M, Kim M, Kwon R, Hwang J, Park K
      Lipids Health Dis, 2015;14(0):116.
      Species: Rat
      Sample Type: Serum
    4. Non-invasive technology that improves cardiac function after experimental myocardial infarction: Whole Body Periodic Acceleration (pGz).
      Authors: Uryash A, Bassuk J, Kurlansky P, Altamirano F, Lopez J, Adams J
      PLoS ONE, 2015;10(3):e0121069.
      Species: Rat
      Sample Type: Tissue Homogenates
    5. Blocking KV1.3 channels inhibits Th2 lymphocyte function and treats a rat model of asthma.
      Authors: Koshy S, Huq R, Tanner M, Atik M, Porter P, Khan F, Pennington M, Hanania N, Corry D, Beeton C
      J Biol Chem, 2014;289(18):12623-32.
      Species: Rat
      Sample Type: BALF
    6. Preventive effects of probiotic bacteria Lactobacillus plantarum and dietary fiber in chemically-induced mammary carcinogenesis.
      Authors: Kassayova M, Bobrov N, Strojny L, Kiskova T, Mikes J, Demeckova V, Orendas P, Bojkova B, Pec M, Kubatka P, Bomba A
      Anticancer Res, 2014;34(9):4969-75.
      Species: Rat
      Sample Type: Serum
    7. Atrial fibrosis in a chronic murine model of obstructive sleep apnea: mechanisms and prevention by mesenchymal stem cells.
      Authors: Ramos P, Rubies C, Torres M, Batlle M, Farre R, Brugada J, Montserrat J, Almendros I, Mont L
      Respir Res, 2014;15(0):54.
      Species: Rat
      Sample Type: Plasma
    8. Improvement of spinal non-viral IL-10 gene delivery by D-mannose as a transgene adjuvant to control chronic neuropathic pain.
      Authors: Dengler E, Alberti L, Bowman B, Kerwin A, Wilkerson J, Moezzi D, Limanovich E, Wallace J, Milligan E
      J Neuroinflammation, 2014;11(0):92.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. S100A1 is released from ischemic cardiomyocytes and signals myocardial damage via Toll-like receptor 4.
      Authors: Rohde D, Schon C, Boerries M, Didrihsone I, Ritterhoff J, Kubatzky K, Volkers M, Herzog N, Mahler M, Tsoporis J, Parker T, Linke B, Giannitsis E, Gao E, Peppel K, Katus H, Most P
      EMBO Mol Med, 2014;6(6):778-94.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    10. Rhamnogalacturonan from Acmella oleracea (L.) R.K. Jansen: gastroprotective and ulcer healing properties in rats.
      Authors: Maria-Ferreira D, da Silva L, Mendes D, Cabrini D, Nascimento A, Iacomini M, Cipriani T, Santos A, Werner M, Baggio C
      PLoS ONE, 2014;9(1):e84762.
      Species: Rat
      Sample Type: Tissue Culture Supernates
    11. Early-life experience decreases drug-induced reinstatement of morphine CPP in adulthood via microglial-specific epigenetic programming of anti-inflammatory IL-10 expression.
      Authors: Schwarz JM, Hutchinson MR, Bilbo SD
      J. Neurosci., 2011;31(49):17835-47.
      Species: Rat
      Sample Type: Serum
    12. Curcumin protects intestinal mucosal barrier function of rat enteritis via activation of MKP-1 and attenuation of p38 and NF-kappaB activation.
      Authors: Song WB, Wang YY, Meng FS, Zhang QH, Zeng JY, Xiao LP, Yu XP, Peng DD, Su L, Xiao B, Zhang ZS
      PLoS ONE, 2010;5(9):e12969.
      Species: Rat
      Sample Type: Plasma
    13. Vipera aspis venom reduces lethality and down-regulates tumor necrosis factor-alpha in a rat model of LPS-induced sepsis.
      Authors: Frolkis I, Klein Y, Locker C, Adi N, Dahan E, Uretzsky G, Shapira I, Sorkine P
      Cytokine, 2010;49(3):319-24.
      Species: Rat
      Sample Type: Serum
    14. Oridonin promotes CD4+/CD25+ Treg differentiation, modulates Th1/Th2 balance and induces HO-1 in rat splenic lymphocytes.
      Authors: Hu AP, Du JM, Li JY, Liu JW
      Inflamm. Res., 2008;57(4):163-70.
      Species: Rat
      Sample Type: Cell Culture Supernates
    15. Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
      Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
      Invest. Ophthalmol. Vis. Sci., 2008;49(9):3999-4007.
      Species: Rat
      Sample Type: Aqueous humor
    16. Expression of the mu opioid receptor in the human immunodeficiency virus type 1 transgenic rat model.
      Authors: Chang SL, Beltran JA, Swarup S
      J. Virol., 2007;81(16):8406-11.
      Species: Rat
      Sample Type: Serum
    17. Cellular recruitment and cytokine generation in a rat model of allergic lung inflammation are differentially modulated by progesterone and estradiol.
      Authors: de Oliveira AP, Domingos HV, Cavriani G, Damazo AS, Dos Santos Franco AL, Oliani SM, Oliveira-Filho RM, Vargaftig BB, de Lima WT
      Am. J. Physiol., Cell Physiol., 2007;293(3):C1120-8.
      Species: Rat
      Sample Type: BALF
    18. Resuscitation from experimental heatstroke by estrogen therapy.
      Authors: Chen SH, Chang FM, Niu KC, Lin MY, Lin MT
      Crit. Care Med., 2006;34(4):1113-8.
      Species: Rat
      Sample Type: Serum
    19. Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure.
      Authors: Holly MK, Dear JW, Hu X, Schechter AN, Gladwin MT, Hewitt SM, Yuen PS, Star RA
      Kidney Int., 2006;70(3):496-506.
      Species: Rat
      Sample Type: Serum
    20. Effects of gabexate mesilate on serum inflammatory cytokines in rats with acute necrotizing pancreatitis.
      Authors: Chen CC, Wang SS, Tsay SH, Lee FY, Lu RH, Chang FY, Lee SD
      Cytokine, 2006;33(2):95-9.
      Species: Rat
      Sample Type: Serum
    21. Immunotherapy of murine prostate cancer using whole tumour cells killed ex vivo by cytosine deaminase/5-fluorocytosine suicide-gene therapy.
      Authors: Pandha HS, Cook D, Greenhalgh R, Dalgleish A
      BJU Int., 2005;95(9):1336-43.
      Species: Rat
      Sample Type: Cell Culture Supernates
    22. Effects of pyrrolidine dithiocarbamate, an NF-kappaB inhibitor, on cytokine expression and ocular inflammation in experimental autoimmune anterior uveitis.
      Authors: Fang IM, Yang CH, Lin CP, Yang CM, Chen MS
      J Ocul Pharmacol Ther, 2005;21(2):95-106.
      Species: Rat
      Sample Type: Aqueous humor
    23. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
      Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
      Crit. Care Med., 2005;33(10):2317-22.
      Species: Rat
      Sample Type: BALF
    24. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
      Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
      J. Immunol., 2005;174(4):2327-35.
      Species: Rat
      Sample Type: Cell Culture Supernates
    25. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
      Authors: Schubert T, Horch RE
      Ann. Surg., 2004;240(2):340-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    26. Ameliorating effects of the immunomodulator 3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole in an experimental model of colitis in the rat.
      Authors: Stasi MA, Ruggiero V, Ursillo A, Taurelli R, Agliano M, Weber E, Lorenzoni P, Sorrentino V, Pacifici L, Carminati P
      Eur. J. Pharmacol., 2004;494(2):263-72.
      Species: Rat
      Sample Type: Tissue Homogenates
    27. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
      Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
      Endocrinology, 2004;145(9):4084-93.
      Species: Rat
      Sample Type: Tissue Homogenates
    Expand to show all 27 Citations
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Rat IL-10 Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed

    Feedback

    Customer Information