Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Antibody was raised against a 17 amino acid peptide near the center of human IL-22. The immunogen is located within amino acids 90 - 140 of IL-22. Amino Acid Squence: EEVLFPQSDRFQPYMQE

Specificity

Multiple isoforms of IL-22 are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit IL-22 Antibody - BSA Free (NBP2-41245) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IL-22 Antibody - BSA Free

Western Blot: IL-22 AntibodyBSA Free [NBP2-41245]

Western Blot: IL-22 AntibodyBSA Free [NBP2-41245]

Western Blot: IL-22 Antibody [NBP2-41245] - Analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: IL-22 Antibody - BSA Free [NBP2-41245]

Immunocytochemistry/ Immunofluorescence: IL-22 Antibody - BSA Free [NBP2-41245]

Immunocytochemistry/Immunofluorescence: IL-22 Antibody [NBP2-41245] - Immunocytochemistry of IL-22 in HeLa cells with IL-22 antibody at 5 ug/mL.
IL-22 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IL-22 Antibody - BSA Free [NBP2-41245] -

Immunocytochemistry/ Immunofluorescence: IL-22 Antibody - BSA Free [NBP2-41245] - Immunofluorescence of IL-22 in HeLa cells with IL-22 antibody at 20 ug/mL.

Applications for IL-22 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/ml

Western Blot

0.5 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP2-41245 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-22

Interleukin-22 (IL-22 or IL-TIF (IL-10-related T-cell derived inducible factor)) belongs to the IL-10 family of regulatory cytokines which also includes IL-10, IL-19, IL-20, IL-24 and IL-26. IL-22 is produced by T lymphocytes following anti-CD3 stimulation. It inhibits IL-4 production of Th2 cells, activates STAT-1 and STAT-3 in several hepatoma cell lines and upregulates the production of acute phase proteins. It may also be involved in inflammatory responses. Human and mouse IL-22 share 79% amino acid identity. Recombinant human IL-22 is a non-disulfide linked homodimer, signaling through a receptor complex of two subunits, IL-22R and IL-10R-beta, belonging to the class II cytokine receptor family.

Long Name

Interleukin 22

Alternate Names

IL-TIF, IL22

Gene Symbol

IL22

Additional IL-22 Products

Product Documents for IL-22 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IL-22 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for IL-22 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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  • IL-22 Antibody - BSA Free
    Name: Kamal Baral
    Application: Western Blot
    Sample Tested: Mouse Liver Tissue
    Species: Mouse
    Verified Customer | Posted 10/21/2025
    Mouse liver lysates were probed with IL-22 antibody at dilution 1:1000 in 1X TBST.
    IL-22 Antibody - BSA Free NBP2-41245

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Protocols

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