ILK Antibody (4F10) - Azide and BSA Free

Novus Biologicals | Catalog # H00003611-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F10

Format

Azide and BSA Free
View all ILK Primary Antibodies »

Product Specifications

Immunogen

ILK (AAH01554, 341 a.a. ~ 452 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQDK

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

ILK - integrin-linked kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ILK Antibody (4F10) - Azide and BSA Free

Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01] - Analysis of ILK expression in transfected 293T cell line by ILK monoclonal antibody (M01), clone 4F10.Lane 1: ILK transfected lysate(51.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ILK Antibody (4F10) [H00003611-M01]

Immunocytochemistry/ Immunofluorescence: ILK Antibody (4F10) [H00003611-M01]

Immunocytochemistry/Immunofluorescence: ILK Antibody (4F10) [H00003611-M01] - Analysis of monoclonal antibody to ILK on Jurkat cell. Antibody concentration 10 ug/ml.
Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01] - Analysis of ILK over-expressed 293 cell line, cotransfected with ILK Validated Chimera RNAi ( Cat # H00003611-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ILK monoclonal antibody (M01) clone 4F10 (Cat # H00003611-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10 Analysis of ILK expression in HeLa.
Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10. Analysis of ILK expression in PC-12.
Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10. Analysis of ILK expression in HepG2.
Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10. Analysis of ILK expression in Raw 264.7.
Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01]

Western Blot: ILK Antibody (4F10) [H00003611-M01] - ILK monoclonal antibody (M01), clone 4F10. Analysis of ILK expression in NIH/3T3.
Sandwich ELISA: ILK Antibody (4F10) [H00003611-M01] - Detection limit for recombinant GST tagged ILK is approximately 3ng/ml as a capture antibody.

Applications for ILK Antibody (4F10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ILK

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Long Name

Integrin-linked Kinase

Alternate Names

ESTM24, p59

Entrez Gene IDs

3611 (Human)

Gene Symbol

ILK

OMIM

602366 (Human)

UniProt

AAH01554 (Human)

Additional ILK Products

Product Documents for ILK Antibody (4F10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ILK Antibody (4F10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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