Influenza A H1N1 PB1 Antibody

Novus Biologicals | Catalog # NBP2-42877

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Virus

Cited:

Porcine

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Reactivity Notes

Influenza A virus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Influenza A H1N1 PB1 Antibody

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 PB1 Antibody [NBP2-42877]

Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 PB1 Antibody [NBP2-42877]

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 PB1 Antibody [NBP2-42877] - Analysis of A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: PB1 protein stained by Influenza A H1N1 PB1 antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37-C,30 min.
Western Blot: Influenza A H1N1 PB1 Antibody [NBP2-42877]

Western Blot: Influenza A H1N1 PB1 Antibody [NBP2-42877]

Western Blot: Influenza A H1N1 PB1 Antibody [NBP2-42877] - Analysis of :
A. 5 ug DF1 whole cell lysate/extract (untreated)
B. 5 ug DF1 whole cell lysate/extract (WSN virus treatment for 10hr) 7.5 % SDS-PAGE Influenza A H1N1 PB1 antibody.
Influenza A H1N1 PB1 Antibody

Western Blot: Influenza A H1N1 PB1 Antibody [NBP2-42877] -

Non-infected (-) and infected (+) H1299 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody (NBP2-42877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Influenza A H1N1 PB1 Antibody

Western Blot: Influenza A H1N1 PB1 Antibody [NBP2-42877] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Influenza A H1N1 PB1 Antibody

Western Blot: Influenza A H1N1 PB1 Antibody [NBP2-42877] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Influenza A H1N1 PB1 Antibody

Western Blot: Influenza A H1N1 PB1 Antibody [NBP2-42877] -

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Influenza A H1N1 PB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Influenza A H1N1 PB1

Alternate Names

Influenza PB1

Additional Influenza A H1N1 PB1 Products

Product Documents for Influenza A H1N1 PB1 Antibody

Certificate of Analysis

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Product Specific Notices for Influenza A H1N1 PB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Influenza A H1N1 PB1 Antibody

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Protocols

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