INTS11 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03680

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all INTS11 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 331-600 of human INTS11 (NP_060341.2). GQSLQIFRKWAGNEKNMVIMPGYCVQGTVGHKILSGQRKLEMEGRQVLEVKMQVEYMSFSAHADAKGIMQLVGQAEPESVLLVHGEAKKMEFLKQKIEQELRVNCYMPANGETVTLPTSPSIPVGISLGLLKREMAQGLLPEAKKPRLLHGTLIMKDSNFRLVSSEQALKELGLAEHQLRFTCRVHLHDTRKEQETALRVYSHLKSVLKDHCVQHLPDGSVTVESVLLQAAAPSEDPGTKVLLVSWTYQDEELGSFLTSLLKKGLPQAPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for INTS11 Antibody - BSA Free

Western Blot: INTS11 AntibodyBSA Free [NBP3-03680]

Western Blot: INTS11 AntibodyBSA Free [NBP3-03680]

Western Blot: INTS11 Antibody [NBP3-03680] - Analysis of extracts of various cell lines, using INTS11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Immunoprecipitation: INTS11 Antibody - BSA Free [NBP3-03680]

Immunoprecipitation: INTS11 Antibody - BSA Free [NBP3-03680]

Immunoprecipitation: INTS11 Antibody [NBP3-03680] - Analysis of 300ug extracts of HeLa cells using 3ug CPSF3L antibody. Western blot was performed from the immunoprecipitate using CPSF3L antibody at a dilition of 1:500.
INTS11 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: INTS11 Antibody - BSA Free [NBP3-03680] -

Immunocytochemistry/ Immunofluorescence: INTS11 Antibody - BSA Free [NBP3-03680] - Immunofluorescence analysis of HeLa cells using INTS11 Rabbit pAb (A6566). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for INTS11 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: INTS11

INT11 has been identified as a component of the twelve-subunit integrator complex that functions in RNA Pol II mediated 3'-end mRNA processing of the U1 and U2 small nuclear RNAs (snRNAs). INT11 is a beta-lactamase family member that has sequence similarity to the cleavage and polyadenylation specificity factors CPSF-73 and CPSF-100.

Alternate Names

cleavage and polyadenylation specific factor 3-like, Cleavage and polyadenylation-specific factor 3-like protein, CPSF3-like protein, EC 3.1.27, EC 3.1.27.-, FLJ20542, INT11, integrator complex subunit 11, INTS11FLJ13294, Protein related to CPSF subunits of 68 kDa, RC68, RC-68CPSF73L, related to CPSF subunits 68 kDa

Gene Symbol

INTS11

Additional INTS11 Products

Product Documents for INTS11 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for INTS11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for INTS11 Antibody - BSA Free

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Protocols

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