IP3R3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03498

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IP3R3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1870 of human IP3R3 (NP_002215.2). EGSEAYQRCESGGFLSKLIQHTKDLMESEEKLCIKVLRTLQQMLLKKTKYGDRGNQLRKMLLQNYLQNRKSTSRGDLPDPIGTGLDPDWSAIAATQCRLDKEGATKLVCDLITSTKNEKIFQESIGLAIHLLDGGNTEIQKSFHNLMMSDKKSERFFKVLHDRMKRAQQETKSTVAVNMNDLGSQPHEDREPVDPTTKGRVASFSIPGSSSRYSLGPSLRRGHEVSERVQSSEMGTSVLIM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

304 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IP3R3 Antibody - BSA Free

IP3R3 Antibody - Azide and BSA Free

Immunoprecipitation: IP3R3 Antibody [NBP3-03498] -

Immunoprecipitation: IP3R3 Antibody [NBP3-03498] - Analysis of 300 μg extracts of Hela cells using 3 μg ITPR3 antibody. Western blot was performed from the immunoprecipitate using ITPR3 antibody at a dilution of 1:1000.
IP3R3 Antibody - Azide and BSA Free

Western Blot: IP3R3 Antibody [NBP3-03498] -

Western Blot: IP3R3 Antibody [NBP3-03498] - Analysis of lysates from HeLa cells, using ITPR3 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.
IP3R3 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: IP3R3 Antibody - Azide and BSA Free [IP3R3] -

Immunocytochemistry/ Immunofluorescence: IP3R3 Antibody - Azide and BSA Free [IP3R3] - Immunofluorescence analysis of NIH-3T3 cells using IP3R3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for IP3R3 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IP3R3

IP3R3 (inositol 1,4,5-triphosphate receptor type 3) is an intracellular calcium release channel that mediates calcium release from the endoplasmic reticulum. There are 3 mammalian IP3R receptors: IP3R1, IP3R2, and IP3R3. IP3R3 has been shown to play a role in olfactory mucus secretion and in the taste perception of sweet, savory (umami), and bitter substances.

Long Name

Inositol 1,4,5-trisphosphate receptor type 3

Alternate Names

InsP3R3, IP3R 3, ITPR3

Gene Symbol

ITPR3

Additional IP3R3 Products

Product Documents for IP3R3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IP3R3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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