Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)

Novus Biologicals | Catalog # NBP3-51942

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2095
View all Legumain/Asparaginyl Endopeptidase Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Legumain/Asparaginyl Endopeptidase (Uniprot: Q99538)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)

Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)

Flow Cytometry: Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)[NBP3-51942] -

Flow cytometric analysis of Legumain/Asparaginyl Endopeptidaseexpression in HeLa cells using Legumain/Asparaginyl Endopeptidase antibody (1:2,000). Green, isotype control; red, Legumain.
Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)

Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)[NBP3-51942] -

Western blotting analysis using Legumain/Asparaginyl Endopeptidase antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Legumain/Asparaginyl Endopeptidase

Legumain encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq]

Alternate Names

AEP, Asparaginyl Endopeptidase, LGMN

Gene Symbol

LGMN

Additional Legumain/Asparaginyl Endopeptidase Products

Product Documents for Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Legumain/Asparaginyl Endopeptidase Antibody (23GB2095)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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