LIGHT/TNFSF14 Antibody

Novus Biologicals | Catalog # NBP1-51981

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LIGHT/TNFSF14 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HEVNPAAHLTGANS corresponding to internal region according to NP_003798.2, NP_742011.1.

Epitope

C-HEVNPAAHLTGANS

Specificity

This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat LIGHT/TNFSF14 Antibody (NBP1-51981) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-LIGHT/TNFSF14 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for LIGHT/TNFSF14 Antibody

Western Blot: LIGHT/TNFSF14 Antibody [NBP1-51981]

Western Blot: LIGHT/TNFSF14 Antibody [NBP1-51981]

Western Blot: LIGHT/TNFSF14 Antibody [NBP1-51981] - Staining of human cerebellum (A) and frontal cortex (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: LIGHT/TNFSF14 Antibody [NBP1-51981]

Immunocytochemistry/ Immunofluorescence: LIGHT/TNFSF14 Antibody [NBP1-51981]

Immunocytochemistry/Immunofluorescence: LIGHT/TNFSF14 Antibody [NBP1-51981] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for LIGHT/TNFSF14 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/mL
Application Notes
WB: Approx. 22 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 22.4 kDa band according to NP_742011.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIGHT/TNFSF14

The T5-39 antibody recognizes human LIGHT (CD258) also known as ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, TR2 ligand, and tumor necrosis factor ligand superfamily member 14 (TNFSF14). LIGHT is a type 2 transmembrane protein, and a member of the tumor necrosis factor ligand superfamily. LIGHT can be proteolytically cleaved and two isofoms have been shown to be produced by alternative splicing. The apparent molecular weight of LIGHT us approximately 30 kD. LIGHT expression has been observed in spleen, brain and kidney; expression is upregulated on activated T cells (particularly CD8+ cells). LIGHT stimulates proliferation of T cells and inhibits growth of some tumor cells. This protein has been shown to play a role in intestinal inflammation and contribute to IgA nephropathy. LIGHT is a ligand for HVEM, and LTbetaR and has also been shown to bind DcR3 and modulate function. In addition, LIGHT has been shown to interact with other proteins including TRAF2, TRAF3, and DIABLO. The T5-39 antibody has been shown to be useful for flow cytometry and ELISA.

Long Name

TNF Ligand Superfamily Member 14

Alternate Names

CD258, TNFSF14

Entrez Gene IDs

8740 (Human)

Gene Symbol

TNFSF14

UniProt

NP_003798.2 (Human)

Additional LIGHT/TNFSF14 Products

Product Documents for LIGHT/TNFSF14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LIGHT/TNFSF14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LIGHT/TNFSF14 Antibody

Customer Reviews for LIGHT/TNFSF14 Antibody

There are currently no reviews for this product. Be the first to review LIGHT/TNFSF14 Antibody and earn rewards!

Have you used LIGHT/TNFSF14 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies