LinkAble™ APC Antibody Conjugation Kits

LinkAble™ APC Antibody Conjugation Kits enable rapid, in house antibody labeling and conjugation to allophycocyanin (APC) through primary amine groups (e.g. lysines). Designed for straightforward, in-house use, the APC conjugation kit supports reliable, reproducible conjugation with minimal hands-on time and no requirement for specialized expertise or additional purification steps. Conjugates are ready to use in approximately two hours, supporting efficient evaluation and comparison of antibodies in downstream assays.

The kits are optimized for single-use reactions and are also available in multipack formats to support higher-throughput or repeat experimental workflows.

Key Benefits

• Ease & Efficiency: Streamlined protocols ensure a simple, fast, and reliable conjugation process. The kit requires only 30 seconds of hands-on time and produces conjugates that are ready to use in 2 hours, without any loss of antibody associated with buffer exchange and purification.
• Reproducibility: Optimized reagents deliver consistent, high-quality results. The kit is available in 25 µg, 100 µg, and 1 mg antibody input sizes, which have been optimized to perform identically across all three sizes, allowing scaling of conjugation reactions.
• Streamlined & Optimized Usability: The ready-to-use format eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience, and reduces the risk of erroneous calculations. An intuitive, user friendly design includes screw cap vials that can be easily centrifuged to recover the sample.

Starting Mass & Volume of Antibody

The kit produces high quality conjugates using IgG antibodies with a concentration of 250 µg/mL or greater. For optimal results, it is recommended to carry out the conjugation reaction with antibody concentration at 1 mg/mL.

Technical Hints & Limitations

• Although it is recommended to use a purified antibody in a suitable buffer (e.g. PBS) and free from additives, the LinkAble™ APC Antibody Conjugation Kit produces high quality conjugates in the presence of some buffers/additives.
  Note: The kit is not compatible with certain components, as described in the table below.

• If multiple, non-optimal parameters are used in the conjugation reaction (concentration, additives, etc.) poor quality conjugates may result.
• Centrifuge all vials before resuspending to ensure all contents are at the bottom of the vials.
• In each step, gently pipette the sample up and down  to mix.
• Purification of the conjugated antibody is not necessary, though a user may choose to do so.