MARK2 Antibody (3B12) - Azide and BSA Free

Novus Biologicals | Catalog # H00002011-M01

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3B12

Format

Azide and BSA Free
View all MARK2 Primary Antibodies »

Product Specifications

Immunogen

MARK2 (AAH08771, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPG

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

MARK2 - MAP/microtubule affinity-regulating kinase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MARK2 Antibody (3B12) - Azide and BSA Free

Western Blot: MARK2 Antibody (3B12) [H00002011-M01]

Western Blot: MARK2 Antibody (3B12) [H00002011-M01]

Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in NIH/3T3.
Western Blot: MARK2 Antibody (3B12) [H00002011-M01]

Western Blot: MARK2 Antibody (3B12) [H00002011-M01]

Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in PC-12.
Western Blot: MARK2 Antibody (3B12) [H00002011-M01]

Western Blot: MARK2 Antibody (3B12) [H00002011-M01]

Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in A-431.
ELISA: MARK2 Antibody (3B12) [H00002011-M01]

ELISA: MARK2 Antibody (3B12) [H00002011-M01]

ELISA: MARK2 Antibody (3B12) [H00002011-M01] - Detection limit for recombinant GST tagged MARK2 is approximately 0.03ng/ml as a capture antibody.

Applications for MARK2 Antibody (3B12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 29941476).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MARK2

EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed.

Alternate Names

EC 2.7.11, EC 2.7.11.1, ELKL motif kinase, ELKL motif kinase 1, EMK-1, EMK1PAR-1, MAP/microtubule affinity-regulating kinase 2MGC99619, PAR1 homolog, Par1b, Ser/Thr protein kinase PAR-1B, serine/threonine protein kinase EMK, serine/threonine-protein kinase MARK2

Entrez Gene IDs

2011 (Human)

Gene Symbol

MARK2

OMIM

600526 (Human)

UniProt

AAH08771 (Human)

Additional MARK2 Products

Product Documents for MARK2 Antibody (3B12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MARK2 Antibody (3B12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MARK2 Antibody (3B12) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MARK2 Antibody (3B12) - Azide and BSA Free

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  • Q: I am interested in MARK2 antibody (3B12), and I would like to know how you have verified its specificity. Did you block with antigen peptide? Or, has it been verified in transfected cells, knockout cell, or siRNA cells?

    A: The MARK2 antibody was validated in the following cell lysates: PC-12, A-431 and NIH/3T3 as well as validated against recombinant protein/immunogen.

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