MDA5 Antibody (SR1076) - BSA Free

Novus Biologicals | Catalog # NBP3-21840

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1076 expressed in HEK293

Format

BSA Free
View all IFIH1/MDA5 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human MDA5 (Uniprot #: Q9BYX4)

Specificity

Detects endogenous levels of total MDA5

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MDA5 Antibody (SR1076) - BSA Free

MDA5 Antibody (SR1076) - BSA Free Western Blot Shows MDA5 Specificity Using knockdown Cell Line.

Western Blot Shows MDA5 Specificity Using knockdown Cell Line.

Western blot shows lysates of DMS 53 parental cell line and MDA5 knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with MDA5 Antibody (SR1076) (Catalog # NBP3-21840) followed by HRP-conjugated secondary antibody. A specific band was detected for MDA5 at approximately 116.6 kDa (as indicated) in the parental DMS 53 cell line, but is not detectable in knockdown DMS 53 cell line. Primary antibody dilution used: 1/500. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] -

Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] - Western blot analysis using NBP3-21840 in THP1 cell lysate.

Applications for MDA5 Antibody (SR1076) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Knockdown Validated

Knockdown Validated from YCharOS Inc. (YCharOS.com)

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFIH1/MDA5

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Long Name

Melanoma Differentiation-Associated Protein 5

Alternate Names

AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1

Gene Symbol

IFIH1

Additional IFIH1/MDA5 Products

Product Documents for MDA5 Antibody (SR1076) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MDA5 Antibody (SR1076) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

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Protocols

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