MED15 Antibody (4A4) - Azide and BSA Free

Novus Biologicals | Catalog # H00051586-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4A4

Format

Azide and BSA Free
View all MED15 Primary Antibodies »

Product Specifications

Immunogen

PCQAP (NP_056973, 1 a.a. ~ 88 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIHNKKSQASVSDPMNALQSL

Specificity

PCQAP - PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MED15 Antibody (4A4) - Azide and BSA Free

Western Blot: MED15 Antibody (4A4) [H00051586-M02]

Western Blot: MED15 Antibody (4A4) [H00051586-M02]

Western Blot: MED15 Antibody (4A4) [H00051586-M02] - Western Blot analysis of PCQAP expression in NIH/3T3(Cat # L018V1 ).
Immunocytochemistry/ Immunofluorescence: MED15 Antibody (4A4) [H00051586-M02]

Immunocytochemistry/ Immunofluorescence: MED15 Antibody (4A4) [H00051586-M02]

Immunocytochemistry/Immunofluorescence: MED15 Antibody (4A4) [H00051586-M02] - Analysis of monoclonal antibody to PCQAP on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: MED15 Antibody (4A4) [H00051586-M02]

Western Blot: MED15 Antibody (4A4) [H00051586-M02]

Western Blot: MED15 Antibody (4A4) [H00051586-M02] - Western Blot analysis of PCQAP expression in Hela NE ( Cat # L013V3 ).
Sandwich ELISA: MED15 Antibody (4A4) [H00051586-M02] - Detection limit for recombinant GST tagged MED15 is 0.03 ng/ml as a capture antibody.

Applications for MED15 Antibody (4A4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MED15

The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARC105Arc105, CAG7A, CTG repeat protein 7a, CTG7A, FLJ42282, FLJ42935, mediator complex subunit 15PC2 glutamine/Q-rich-associated protein, mediator of RNA polymerase II transcription subunit 15, PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associatedprotein, PC2-glutamine-rich-associated protein, PCQAPDKFZp686A2214, Positive cofactor 2 glutamine/Q-rich-associated protein, positive cofactor 2, glutamine/Q-rich-associated protein, TIG1DKFZp762B1216, TIG-1TPA-inducible gene 1 protein, TNRC7, TPA inducible gene-1, TPA inducible protein, trinucleotide repeat containing 7,105-kD, Trinucleotide repeat-containing gene 7 protein

Entrez Gene IDs

51586 (Human)

Gene Symbol

MED15

OMIM

607372 (Human)

UniProt

NP_056973 (Human)

Additional MED15 Products

Product Documents for MED15 Antibody (4A4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MED15 Antibody (4A4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MED15 Antibody (4A4) - Azide and BSA Free

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Protocols

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FAQs

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