Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Kappa/IgG2a Kappa/IgG1 Kappa Clone # A103 + T311 + HMB45

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Localization

Cytoplasmic

Marker

Melanoma Marker

Specificity

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Kappa/IgG2a Kappa/IgG1 Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34339).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45)Azide and BSA Free [NBP2-34547]

Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45)Azide and BSA Free [NBP2-34547]

Western Blot: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free [NBP2-34547] - Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker (MART-1 + Tyrosinase + gp100) MAb (A103 + T311 + HMB45).
Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free [NBP2-34547]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free [NBP2-34547]

Immunohistochemistry-Paraffin: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free [NBP2-34547] - Formalin-fixed, paraffin-embedded human melanoma stained with Melanoma Marker MAb (A103 + T311 + HMB45).

Applications

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Western Blot

2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Melanoma Marker (MART-1 + Tyrosinase + gp100)

Alternate Names

MART1, MART-1, melan-A, MLANA

Gene Symbol

MLANA

Additional Melanoma Marker (MART-1 + Tyrosinase + gp100) Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (A103 + T311 + HMB45) - Azide and BSA Free

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Protocols

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