Human MMP-2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA0391

Novus Biologicals
Discontinued Product
KA0391 has been discontinued. View all MMP-2 products.

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Plasma (Heparin), Serum (100 uL)

Sensitivity

<10 pg/mL

Assay Range

625 to 40000 pg/mL
View all MMP-2 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

This ELISA kit has been transitioned to a new version, available here: MMP200

Kit Contents for Human MMP-2 ELISA Kit (Colorimetric)

  • 96-well plate precoated with anti- human MMP-2 antibody.
  • ABC diluent buffer
  • Antibody diluent buffer
  • Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100.
  • Biotinylated anti- human MMP-2 antibody, dilution 1:100.
  • Lyophilized recombinant human MMP-2 standard
  • Sample diluent buffer
  • TMB color developing agent
  • TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-2

MMP2 is a member of the Matrix Metalloproteinase (MMP) family, which are involved in extracellular matrix degradation under both normal physiological and disease processes. Specifically, MMP-2 is responsible for degrading collagens type IV, V, VII, and X and gelatin type I. Other reported functions of MMP 2 include angiogenesis, tumor invasion, tissue repair, remodeling of the vasculature, inflammation, and atherosclerotic plaque rupture. MMP-2 secretion is activated by the Ras signaling pathway. MMP2 triggers cells to begin migration by cleaving laminin-5 and exposing an integrin-binding site on the epithelial basement membrane. The cleaved of laminin has been detected in tumors and in tissues being altered, howver the activated form of MMP2 is not found in benign tumors. Thus, detection of MMP2 is a possible early indicator of tumor activity. MMP2 is most strongly expressed in normal skin fibroblasts, but may also be found in gliomas, breast and prostate tumors. Mutations in the MMP-2 gene have been linked to Torg-Winchester syndrome, Nodulosis-Arthropathy-Osteolysis (NAO) syndrome, and arthritis. Additionally, abnormal expression of matrix matalloproteinases have been associated with neoplastic traits such as loss of negative growth regulation and high invasive potential.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for Human MMP-2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human MMP-2 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Human MMP-2 ELISA Kit (Colorimetric)

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FAQs for Human MMP-2 ELISA Kit (Colorimetric)

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  • Q: I have some questions about MMP-2 kit KA0391 and TMP-2 kit KA0549. What are they measuring? The active form the pro-form and/or the complex. Can I use EDTA-plasma?

    A: Both of these kits are produced by a Taiwanese company called Abnova and we distribute for them. KA0391 detects both the zymogen and active enzyme and is suitable for body fluid, cell culture supernatant, plasma, serum and tissue lysate samples. KA0549 can detect TIMP2 sera, plasma, body fluids, tissue lysates or cell culture supernatants and Abnova does not distinguish between detecting different forms, so it will probably work for all.

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