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Total MMP-2 Quantikine ELISA Kit

R&D Systems | Catalog # MMP200

For human, mouse, rat, porcine, and canine samples.
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.082 ng/mL

Assay Range

0.5-32 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Total MMP-2 Quantikine ELISA Kit

The Quantikine Total MMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MMP-2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse/rat pro-MMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural MMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MMP-2.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human, Mouse, Rat, Porcine, Canine

Specificity

Recombinant MMP-2 and natural human, mouse, rat, porcine, and canine active, pro-, and TIMP complexed MMP-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.30 11.2 22.8 4.26 11.1 21.5
Standard Deviation 0.302 0.420 0.828 0.275 0.738 1.51
CV% 7.0 3.8 3.6 6.5 6.6 7.0

Recovery for Total MMP-2 Quantikine ELISA Kit

The recovery of Total MMP-2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 90-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total MMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Linearity

Scientific Data Images for Total MMP-2 Quantikine ELISA Kit

Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Standard Curve

Human/Mouse/Rat/Porcine/Canine Total MMP-2 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-2

MMP-2 (matrix metalloproteinase 2), also known as Gelatinase A, is upregulated at sites of tissue damage, inflammation, and in stromal cells surrounding the invading front of metastatic tumors. The latent proenzyme (proMMP-2) is activated by MT-MMPs, Thrombin, or activated Protein C. Mature MMP-2 activity is inhibited by alpha 2-Macroglobulin or TIMPs 1, 2, 3, and -4. TIMP-2 and -3 can also tether proMMP-2 into cell surface ternary complexes with MT-MMPs. MMP-2 degrades Gelatin (denatured Collagen) and type IV Collagen, the major component of basement membranes. It can also degrade the matrix proteins Collagens V, VII and X, Decorin, Elastin, and Fibronectin. MMP-2 processes and modulates the functions of many other vasoactive and proinflammatory molecules including Adrenomedullin, Big Endothelin-1, Calcitonin gene-related peptide, CCL7/MCP-3, CXCL12/SDF-1, Galectin-3, IGFBP-3, IL-1 beta, S100A8, and S100A9.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for Total MMP-2 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Total MMP-2 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Total MMP-2 Quantikine ELISA Kit

Customer Reviews for Total MMP-2 Quantikine ELISA Kit (5)

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  • Total MMP-2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma and Urine
    Verified Customer | Posted 03/16/2023
    Tested plasma at 30-fold dilution and all samples were in range. Tested urine at 2-fold dilution and all samples were undetectable.
    Total MMP-2 Quantikine ELISA Kit MMP200
  • Total MMP-2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 09/26/2022
    Total MMP-2 Quantikine ELISA Kit MMP200
  • Total MMP-2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Human Plasma
    Verified Customer | Posted 04/06/2022
    Total MMP-2 Quantikine ELISA Kit MMP200
  • Total MMP-2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: 7TD1 mouse hybridoma cell line
    Verified Customer | Posted 10/26/2021
    Total MMP-2 Quantikine ELISA Kit MMP200
  • Total MMP-2 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Human Aortic Endothelial Cells
    Verified Customer | Posted 03/21/2019

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Protocols

View specific protocols for Total MMP-2 Quantikine ELISA Kit (MMP200):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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