Total MMP-2 Quantikine ELISA Kit

For human, mouse, rat, porcine, and canine samples
  (32 citations)     
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (32)
FAQs
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.082 ng/mL
  • Assay Range
    0.5 - 32 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Recombinant MMP-2 and natural human, mouse, rat, porcine, and canine active, pro-, and TIMP complexed MMP-2.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC126 , Quantikine Immunoassay Control Set 923 for Total MMP-2 - Please Inquire
Product Summary
The Quantikine Total MMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MMP-2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse/rat pro-MMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural MMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MMP-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 4.3 11.2 22.8 4.26 11.1 21.5
Standard Deviation 0.302 0.42 0.828 0.275 0.738 1.51
CV% 7 3.8 3.6 6.5 6.6 7

Recovery

The recovery of Total MMP-2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 90-105
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total MMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 MMP-2 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-2

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

  • Long Name:
    Matrix Metalloproteinase 2
  • Entrez Gene IDs:
    4313 (Human); 17390 (Mouse)
  • Alternate Names:
    72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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Species
Sample Type
  1. Matrix Metalloproteinase-Mediated Blood-Brain Barrier Dysfunction in Epilepsy
    Authors: RG Rempe, AMS Hartz, ELB Soldner, BS Sokola, SR Alluri, EL Abner, RJ Kryscio, A Pekcec, J Schlichtig, B Bauer
    J. Neurosci., 2018;0(0):.
    Species: Rat
    Sample Type: Tissue Homogenates
  2. Specific biomarkers of myocardial inflammation and remodeling processes as predictors of mortality in high-risk patients undergoing percutaneous mitral valve repair (MitraClip)
    Authors: O Dörr, C Walther, C Liebetrau, T Keller, H Tabert, N Boeder, M Bayer, P Bauer, H Möllmann, L Gaede, C Troidl, S Voss, T Bauer, CW Hamm, H Nef
    Clin Cardiol, 2018;0(0):.
    Species: Human
    Sample Type: Serum
  3. miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development
    Authors: J Ma, D Li, FF Kong, D Yang, H Yang, XX Ma
    J. Exp. Clin. Cancer Res., 2018;37(1):19.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. Chebulinic acid inhibits smooth muscle cell migration by suppressing PDGF-R? phosphorylation and inhibiting matrix metalloproteinase-2 expression
    Authors: IS Song, YJ Jeong, JH Park, S Shim, SW Jang
    Sci Rep, 2017;7(1):11797.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Comparison of fibrin glue and Vicryl sutures in conjunctival autografting for pterygium surgery
    Authors: X Wang, Y Zhang, L Zhou, R Wei, L Dong
    Mol. Vis., 2017;23(0):275-285.
    Species: Human
    Sample Type: Tears
  6. TIMP1 and MMP9 are predictors of mortality in septic patients in the emergency department and intensive care unit unlike MMP9/TIMP1 ratio: Multivariate model
    Authors: ME Niño, SE Serrano, DC Niño, DM McCosham, ME Cardenas, VP Villareal, M Lopez, A Pazin-Filh, FA Jaimes, F Cunha, R Schulz, D Torres-Due
    PLoS ONE, 2017;12(2):e0171191.
    Species: Human
    Sample Type: Serum
  7. Suppressive Effect of the n-Hexane Extract of Litsea japonica Fruit Flesh on Monosodium-Iodoacetate-Induced Osteoarthritis in Rats
    Authors: SH Kim, HJ Choi, WK Yang, JE Lee, JH Cho, IJ Park, S Park, BK Park, M Jin
    Evid Based Complement Alternat Med, 2017;2017(0):1791403.
    Species: Rat
    Sample Type: Tissue Homogenates
  8. Down-regulating Myoferlin inhibits the vasculogenic mimicry of�melanoma via decreasing MMP-2 and inducing mesenchymal-to-epithelial transition
    Authors: W Zhang, P Zhou, A Meng, R Zhang, Y Zhou
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Aurora Kinase A is a Biomarker for Bladder Cancer Detection and Contributes to its Aggressive Behavior
    Authors: A Mobley, S Zhang, J Bondaruk, Y Wang, T Majewski, NP Caraway, L Huang, E Shoshan, G Velazquez-, G Nitti, S Lee, JG Lee, E Fuentes-Ma, D Willis, L Zhang, CC Guo, H Yao, K Baggerly, Y Lotan, SP Lerner, C Dinney, D McConkey, M Bar-Eli, B Czerniak
    Sci Rep, 2017;7(0):40714.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. CCAAT/enhancer-binding protein ? overexpression alleviates myocardial remodelling by regulating angiotensin-converting enzyme-2 expression in diabetes
    Authors: Y Tie, C Zhai, Y Zhang, X Qin, F Yu, H Li, M Shan, C Zhang
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Mouse
    Sample Type: Serum
  11. Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo
    Authors: A Bao, KF Che, S Bozinovski, J Ji, JA Gregory, S Kumlien Ge, M Adner, LO Cardell, A Lindén
    PLoS ONE, 2017;12(12):e0188909.
    Species: Mouse
    Sample Type: BALF
  12. Beneficial Effects of Galectin-3 Blockade in Vascular and Aortic Valve Alterations in an Experimental Pressure Overload Model
    Authors: J Ibarrola, E Martínez-M, JR Sádaba, V Arrieta, A García-Peñ, V Álvarez, A Fernández-, A Gainza, P Rossignol, V Cachofeiro, N López-Andr
    Int J Mol Sci, 2017;18(8):.
    Species: Rat
    Sample Type: Tissue Homogenates
  13. CP-25, a novel compound, protects against autoimmune arthritis by modulating immune mediators of inflammation and bone damage
    Sci Rep, 2016;6(0):26239.
    Species: Rat
    Sample Type: Synovial Fluid
  14. Magnobovatol inhibits smooth muscle cell migration by suppressing PDGF-R? phosphorylation and inhibiting matrix metalloproteinase-2 expression
    Authors: H Kang, DH Ahn, JH Pak, KH Seo, NI Baek, SW Jang
    Int J Mol Med, 2016;37(5):1239-46.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Differential diagnosis of lung cancer, its metastasis and chronic obstructive pulmonary disease based on serum Vegf, Il-8 and MMP-9
    Sci Rep, 2016;6(0):36065.
    Species: Human
    Sample Type: Serum
  16. MMP-2 Isoforms in Aortic Tissue and Serum of Patients with Ascending Aortic Aneurysms and Aortic Root Aneurysms
    PLoS ONE, 2016;11(11):e0164308.
    Species: Human
    Sample Type: Serum
  17. Concomitant elevations of MMP-9, NGAL, proMMP-9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
    J. Cell. Mol. Med, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  18. Exosomes in Human Breast Milk Promote EMT
    Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
    Clin Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
  19. The pattern of hMENA isoforms is regulated by TGF-?1 in pancreatic cancer and may predict patient outcome
    Authors: R Melchionna, P Iapicca, F Di Modugno, P Trono, I Sperduti, M Fassan, I Cataldo, BC Rusev, RT Lawlor, MG Diodoro, M Milella, GL Grazi, MJ Bissell, A Scarpa, P Nisticò
    Oncoimmunology, 2016;5(12):e1221556.
    Species: Human
    Sample Type: Cell Culture Supernates
  20. A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease
    Authors: Ilias P Doulamis
    Int. J. Cardiol, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  21. Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.
    Authors: Kmiecik A, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
    PLoS ONE, 2015;10(5):e0124865.
    Species: Human
    Sample Type: Cell Culture Supernates
  22. Aqueous and alcoholic extracts of Triphala and their active compounds chebulagic acid and chebulinic acid prevented epithelial to mesenchymal transition in retinal pigment epithelial cells, by inhibiting SMAD-3 phosphorylation.
    Authors: Sivasankar S, Lavanya R, Brindha P, Angayarkanni N
    PLoS ONE, 2015;10(3):e0120512.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. Effect of mesenchymal stem cells and extracts derived from the placenta on trophoblast invasion and immune responses.
    Authors: Choi, Jong Ho, Jung, Jieun, Na, Kyu-Hwan, Cho, Kyung Ji, Yoon, Tae Ki, Kim, Gi Jin
    Stem Cells Dev, 2014;23(2):132-45.
    Species: Human
    Sample Type: Cell Culture Supernates
  24. Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?
    Authors: Naik, Pooja, Fofaria, Neel, Prasad, Shikha, Sajja, Ravi K, Weksler, Babette, Couraud, Pierre-O, Romero, Ignacio, Cucullo, Luca
    BMC Neurosci, 2014;15(0):51.
    Species: Human
    Sample Type: Cell Culture Supernates
  25. Overexpression of EMMPRIN isoform 2 is associated with head and neck cancer metastasis.
    Authors: Huang Z, Tan N, Guo W, Wang L, Li H, Zhang T, Liu X, Xu Q, Li J, Guo Z
    PLoS ONE, 2014;9(4):e91596.
    Species: Human
    Sample Type: Cell Culture Supernates
  26. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
    Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
    Mol Cancer, 2014;13(0):88.
    Species: Human
    Sample Type: Cell Culture Supernates
  27. Role of serum VEGFA, TIMP2, MMP2 and MMP9 in monitoring response to adjuvant radiochemotherapy in patients with primary cervical cancer--results of a companion protocol of the randomized NOGGO-AGO phase III clinical trial.
    Authors: Braicu E, Gasimli K, Richter R, Nassir M, Kummel S, Blohmer J, Yalcinkaya I, Chekerov R, Ignat I, Ionescu A, Mentze M, Fotopoulou C, Pop C, Lichtenegger W, Sehouli J
    Anticancer Res, 2014;34(1):385-91.
    Species: Human
    Sample Type: Serum
  28. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.
    Species: Mouse
    Sample Type: Tissue Homogenates
  29. Tissue redox activity as a hallmark of carcinogenesis: from early to terminal stages of cancer.
    Authors: Bakalova R, Zhelev Z, Aoki I, Saga T
    Clin Cancer Res, 2013;19(9):2503-17.
    Species: Mouse
    Sample Type: Plasma
  30. Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
    Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
    Cytokine, 2013;61(3):755-8.
    Species: Human
    Sample Type: Serum
  31. Neutrophil-derived matrix metalloproteinase-9 is a potent activator of trypsinogen in acinar cells in acute pancreatitis.
    Authors: Awla D, Abdulla A, Syk I, Jeppsson B, Regner S, Thorlacius H
    J. Leukoc. Biol., 2012;91(5):711-9.
    Species: Mouse
    Sample Type: Plasma
  32. Celastrol suppresses allergen-induced airway inflammation in a mouse allergic asthma model.
    Authors: Kim DY, Park JW, Jeoung D, Ro JY
    Eur. J. Pharmacol., 2009;612(1):98-105.
    Species: Mouse
    Sample Type: BALF
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Quantikine Immunoassay Control Set 923 for Total MMP-2

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