Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

R&D Systems | Catalog # DY2106-05

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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

62.5-4000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all BAFF/BLyS/TNFSF13B ELISA Kits »

Product Summary for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse BAFF / BLyS / TNFSF13B. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

Mouse BAFF / BLyS / TNFSF13B ELISA Standard Curve

Mouse BAFF / BLyS / TNFSF13B ELISA Standard Curve

Kit Contents for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BAFF/BLyS/TNFSF13B

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4

Entrez Gene IDs

10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)

Gene Symbol

TNFSF13B

Additional BAFF/BLyS/TNFSF13B Products

Product Documents for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

For research use only

Citations for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

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  • Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell Lysates
    Verified Customer | Posted 08/10/2019
    Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA DY2106-05
  • Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/08/2018
    Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA DY2106-05

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