Mouse CCL9/10/MIP-1 gamma Antibody (62105)

R&D Systems | Catalog # MAB463

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization, ELISA Development, ELISpot Development, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 62105
View all CCL9/10/MIP-1 gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL9/10/MIP‑1 gamma
Gln22-Gln122
Accession # P51670

Specificity

Detects mouse CCL9/10/MIP‑1 gamma in ELISAs and Western blots. In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human CCL3, 4, 15, 19, 20, recombinant mouse CCL3, 4, 19, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL9/10/MIP-1 gamma Antibody (62105)

Neutralization by Mouse CCL9/10/ MIP 1 gamma Antibody

Mouse CCL9/10/MIP 1 gamma Antibody Clone # 62105 (Catalog # MAB463) neutralizes CCL9/10/MIP-1 gamma (463-MG) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR1. The Neutralization Dose (ND50) for this effect is typically 1.50-15.0 µg/mL in the presence of 1.00 μg/mL Recombinant Mouse CCL9/10/MIP-1 gamma.
Mouse CCL9 / 10 / MIP-1 gamma ELISA Standard Curve

Mouse CCL9 / 10 / MIP-1 gamma ELISA Standard Curve

Recombinant Mouse CCL9/10/MIP‑1 gamma (Catalog # 463-MG) was serially diluted and captured by Rat Anti-Mouse CCL9/10/MIP‑1 gamma Monoclonal Antibody (Catalog # MAB463) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL9/10/MIP‑1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF463) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CCL9/10/MIP-1 gamma Antibody (62105)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL9/10/MIP‑1 gamma (Catalog # 463-MG) under non-reducing conditions only

Neutralization

Mouse CCL9/10/MIP 1 gamma Antibody Clone # 62105 (Catalog # MAB463) neutralizes CCL9/10/MIP-1 gamma (Catalog # 463-MG) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR1. The Neutralization Dose (ND50) for this effect is typically 1.50-15.0 µg/mL in the presence of 1.00 μg/mL Recombinant Mouse CCL9/10/MIP-1 gamma.

Mouse CCL9/10/MIP-1 gamma Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse CCL9/10/MIP‑1 gamma Biotinylated Antibody (Catalog # BAF463)
  • Standard: Recombinant Mouse CCL9/10/MIP-1 gamma Protein (Catalog # 463-MG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL9/10/MIP-1 gamma

Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1‑3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3‑7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full‑length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71‑73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full‑length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).

References

  1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
  2. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
  3. Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
  4. Haelens, A. et al. (1996) Immunobiology 195:499.
  5. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
  6. Youn, B-S. et al. (1995) J. Immunol. 155:2661.
  7. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.

Alternate Names

CCF18, MIP-1 gamma, MIP1 gamma

Entrez Gene IDs

20308 (Mouse)

Gene Symbol

CCL9

UniProt

P51670

Additional CCL9/10/MIP-1 gamma Products

Product Documents for Mouse CCL9/10/MIP-1 gamma Antibody (62105)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL9/10/MIP-1 gamma Antibody (62105)

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Mouse CCL9/10/MIP-1 gamma Antibody (62105)

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