Mouse FGF-21 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF3057G Datasheet / COA / SDS

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AF3057G-100UG

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All FGF-21 Antibodies

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Mouse FGF-21 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF-21 in direct ELISAs and Western blots. In these formats, approximately 15% cross‑reactivity with recombinant human (rh) FGF-21 is observed and less than 1% cross-reactivity with rhFGF-19 and rhFGF-23 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse FGF-21
Tyr30-Ser210
Accession # Q9JJN1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FGF-21

Mouse FGF-21 is a 20 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF family. It is synthesized as a 210 amino acid (aa) precursor that contains a 28 aa signal sequence and a 182 aa mature region. Mature mouse FGF-21 shares 81%, 80%, 79% and 92% aa sequence identity with human, bovine, canine and rat FGF-21, respectively. FGF-21 seems to be produced by the liver and exhibits unique antidiabetogenic effects in fat.

Long Name

Fibroblast Growth Factor 21

Entrez Gene IDs

26291 (Human); 56636 (Mouse)

Alternate Names

FGF21; FGF-21; fibroblast growth factor 21